We narrowed to 1,598 results for: pfas

1. pFA6a-TRP1-PGAL1-3HA

   Plasmid

   #41609

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   GAL1 promoter (GAL1 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   3xHA, TRP1 reverse orientation, and yeast ADH1 te…

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-link-yoPSmOrange-Kan

   Plasmid

   #44951

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPSmOrange
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA CUP1p-NuiHA kanMX

   Plasmid

   #131169

   Purpose

   PCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiae

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   Nui-HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-natMX6-P81nmt1-3FLAG

   Plasmid

   #19347

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Aug 26. ():.

   Insert

   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFA6a-link-yoGFPgamma-SpHis5

   Plasmid

   #44859

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized GFPgamma with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoGFPgamma
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-link-yomEos2-SpHis5

   Plasmid

   #44849

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mEos2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomEos2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac(His10-SNAP-Csk)

   Plasmid

   #177895

   Purpose

   Baculo expression of His10 tag fused to SNAPtag and human Csk

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Csk (CSK Human)
   

   Use

   Tags

   10xHis-TEV-TVMV-SNAP tag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-link-ymVenus-URA3

   Plasmid

   #168058

   Purpose

   Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymVenus
   

   Use

   Tags

   ymVenus

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac HTB DPP9S S730A

   Plasmid

   #169946

   Purpose

   Purification of catalytically-dead DPP9S from insect cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   HIS-TEV

   Expression

   Insect

   Mutation

   S730A

   Promoter

   polyhedrin

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGZ173 (pFA6a-SL-MNase-kanMX6)

    Plasmid

    #70234

    Purpose

    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

    Depositor

    Steven Henikoff

    Article

    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

    Insert

    Short linker and aa 83-231 of Mnase
    

    Use

    Yeast genomic targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac_Dual_GST-TEV-EGFP-TBK1

    Plasmid

    #187830

    Purpose

    Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST and TEV-mEGFP-GG Linker

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac SpyTag-β2AR-SpyTag

    Plasmid

    #135620

    Purpose

    Expression of SpyTag-B2AR in insect cell

    Depositor

    Hee-Jung Choi

    Article

    Choi et al Science. 2019 Nov 29;366(6469):1150-1156

    Insert

    Beta 2 adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

    Expression

    Insect

    Mutation

    Deleted 1-24 aa, 366-413 aa

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-link-yomKeima-SpHis5

    Plasmid

    #44847

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKeima
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-link-yoClover-Kan

    Plasmid

    #44952

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized Clover with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoClover
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-kanMX6-P41nmt1-GFP

    Plasmid

    #39290

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pFA6a-natMX6-Purg1-3FLAG

    Plasmid

    #19355

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Aug 26. ():.

    Insert

    urg1 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-link-yoTagRFP657-CaURA3

    Plasmid

    #44882

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRFP657 with CaUra3 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac HTB DPP9S D808N

    Plasmid

    #169947

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    D808N

    Promoter

    polyhedrin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-link-yomKO2-SpHis5

    Plasmid

    #44845

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mKO2 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomKO2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge