We narrowed to 34,903 results for: CaS;

1. pUPD2 LbCas12a (GB1439)

   Plasmid

   #160573

   Purpose

   Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   LbCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUPD2 AsCas12a (GB1438)

   Plasmid

   #160572

   Purpose

   Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AsCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9H(-10TC)_arr2

   Plasmid

   #149587

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEM-eCas9-D1135E

   Plasmid

   #154926

   Purpose

   aTc inducible eCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   eCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEM-OptiCas9-D1135E

   Plasmid

   #154928

   Purpose

   aTc inducible OptiCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   OptiCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   R661A, K1003H, D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM-HypaCas9-D1135E

   Plasmid

   #154930

   Purpose

   aTc inducible HypaCas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   HypaCas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N692A/M694A/Q695A/H698A/D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEM-Cas9-D1135E

   Plasmid

   #154931

   Purpose

   aTc inducible Cas9 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   Cas9-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   D1135E

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEM-Cas9HF1-D1135E

   Plasmid

   #154924

   Purpose

   aTc inducible Cas9HF1 with D1135E mutation

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   Cas9HF1-D1135E
   

   Use

   CRISPR and Synthetic Biology

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

   Promoter

   pTet

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_eA3A T31A-nCas9

   Plasmid

   #163538

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)eA3A T31A

   Promoter

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_UdgX-EE-UdgX-nCas9

    Plasmid

    #163564

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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