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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 39,152 results for: Eras

Showing: 7471 - 7500 of 39152 results

  1. pSL0762 (pQCascade_crRNA-nt)

    Plasmid
    #130636
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130636-plasmid-map-sequence-id-255017

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  4. pLVX-mCherry-SPASTIN

    Plasmid
    #180647
    Purpose
    Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    SPASTIN
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Starts at M87, has siRNA resistance to GAACAGUGUG…
    Promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180647-plasmid-map-sequence-id-360020

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  8. pBEG R2-iHygro-L3 #BGV206

    Plasmid
    #48985
    Purpose
    Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    hygromycin phosphotransferase
    Use
    Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    na
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. dCas9-NLS-gs10-cTRb413

    Plasmid
    #188261
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cTRb413 (TXNRD2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188261-plasmid-map-sequence-id-374572

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  17. pGL3 Pro Mut E2

    Plasmid
    #48750
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutated E-box2 sequence from CACGTT to GCTAGT
    Promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    48750-plasmid-map-sequence-id-448242

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  19. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

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  20. pmIL-6 mut C/EBP

    Plasmid
    #61291
    Purpose
    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to…
    Promoter
    IL-6 promoter with mutant C/EBP binding site
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. VanGlow-GL

    Plasmid
    #83342
    Purpose
    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Article
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    GFP::Luciferase(nls)
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83342-plasmid-map-sequence-id-158671

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Showing: 7471 - 7500 of 39152 results