We narrowed to 9,825 results for: 158

1. Polymerase expression - pCMV-B103 (LM2907)

   Plasmid

   #208968

   Purpose

   Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. nLuc_AP4_TEVs_P3mS

   Plasmid

   #119299

   Purpose

   Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
   

   Use

   Luciferase

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEos3.2trLAT-2P

   Plasmid

   #203748

   Purpose

   Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trLAToC27AC30A (Lat Mouse)
   

   Use

   Tags

   ER translocation signal - mEos3.2

   Expression

   Mammalian

   Mutation

   C27A, C30A

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-A3A-seBE4max-IRES

   Plasmid

   #174698

   Purpose

   Small-molecule controlled base editing

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

   Insert

   A3A-seBE4max-IRES (APOBEC3A Human)
   

   Use

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   A3A-M13I, UGI-E11K

   Promoter

   CMV

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. WT N-His Ana GvpC in pET28a

   Plasmid

   #153294

   Purpose

   Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   Gas vesicle protein C (N-terminal his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Added a hexahistidine tag after the methionine at…

   Promoter

   T7

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AP4mS_PPVs_P3_cLuc

   Plasmid

   #119300

   Purpose

   Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304_zeo_eGFP_V5

   Plasmid

   #160096

   Purpose

   Expresses eGFP in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX307 ARHGAP30

   Plasmid

   #117738

   Purpose

   Open reading frame vector encoding ARHGAP30

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   ARHGAP30 (ARHGAP30 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-BAP1-S172R-GFP

   Plasmid

   #78918

   Purpose

   Mammalian expression of BAP1 with S172R and an EGFP fusion

   Depositor

   Michael Hendzel

   Article

   Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   S172R

   Promoter

   CMV

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

10. nDHFR173-gs10-NLS-VPR

    Plasmid

    #188262

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nDHFR173 (DHFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FH1-PNGT#1-mCherry

    Plasmid

    #164098

    Purpose

    Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    PNGT#1-mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Puro-SIK2

    Plasmid

    #138696

    Purpose

    Expresses a human SIK2-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK2 human (SIK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-Puro-SIK3

    Plasmid

    #138697

    Purpose

    Expresses a human SIK3-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgSIK3 human (SIK3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nup98-pDONR221

    Plasmid

    #124241

    Purpose

    Gateway entry clone of Drosophila Nup98-96 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Nup98-96 (Nup98-96 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-BAP1-C91W-GFP

    Plasmid

    #78913

    Purpose

    Mammalian expression of BAP1 with C91W and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    C91W

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pSECC-sgLKB1-A

    Plasmid

    #138663

    Purpose

    Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgLKB1 mouse (Stk11 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. [pSK483] pLJC5 Flag-Depdc5(P)

    Plasmid

    #109342

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant P

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    YDLLP(775-779)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. B*07:05:01

    Plasmid

    #203268

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*07:05:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPHK2 gRNA (BRDN0001146242)

    Plasmid

    #77095

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. A*68:01:01

    Plasmid

    #203292

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*68:01:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-FLAG-MTOR-D2512H

    Plasmid

    #69017

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-D2512H (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Aspartate 2512 to Histidine

    Promoter

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pLX311 ARHGAP30

    Plasmid

    #117739

    Purpose

    Open reading frame vector encoding ARHGAP30

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    ARHGAP30 (ARHGAP30 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. [pSK496] pLJC5 HA-Depdc5(AB)

    Plasmid

    #109346

    Purpose

    Lenti-viral expression of HA-tagged Depdc5 mutant AB

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HA-BAP1-N102S-GFP

    Plasmid

    #78915

    Purpose

    Mammalian expression of BAP1 with N102S and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    N102S

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

    Plasmid

    #163644

    Purpose

    Makes a lentivirus that expresses mNeonGreen and Cas9.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    mNeonGreen-P2A-Cas9
    

    Use

    Lentiviral

    Tags

    mNeonGreen-P2A

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-45b-NFYC-IDR-mEGFP

    Plasmid

    #185015

    Purpose

    Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    NFYC IDR (Nfyc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLCHKO_HPRT1_exon3_2_Lb

    Plasmid

    #155054

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid

    #155051

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCR1272

    Plasmid

    #111107

    Purpose

    Expresses FANCI_+_89787428.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCI gRNA (FANCI Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330-NQL007-NANOG-sgRNA

    Plasmid

    #175555

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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