We narrowed to 22,191 results for: his

1. pcDNA3-BAP-Sox2

   Plasmid

   #133281

   Purpose

   Expression of target proteins BAP-Sox2 in mammalian cells

   Depositor

   Arman Kulyyassov

   Article

   Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Tags

   Biotin Acceptor Peptide contains 7-His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEBTetD-SNAP-FOP

   Plasmid

   #136797

   Purpose

   Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-FGFR
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PM18033-PLS-KP6-A66S_rpoA-U

   Plasmid

   #190989

   Purpose

   Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in human cells

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   DYW-KP:A66S

   Promoter

   CMV

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PM18033-PLS-KP2-A66S_rpoA-U

   Plasmid

   #190977

   Purpose

   Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in human cells

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP2
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   DYW-KP:A66S

   Promoter

   CMV

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Pdar

   Plasmid

   #24786

   Depositor

   David Baker

   Article

   Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

   Insert

   Pdar
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pET28b – E. coli RecE residues 564-868

   Plasmid

   #104534

   Purpose

   Expresses E. coli RecE residues 564-868

   Depositor

   Charles Bell

   Article

   Zhang et al Structure. 2009 May 13;17(5):690-702. do

   Insert

   RecE residues 564-866 (C-terminal nuclease domain)
   

   Use

   Tags

   6X-His tag followed by thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YFP-Gamma 5

   Plasmid

   #36044

   Depositor

   Narasimhan Gautam

   Article

   Akgoz et al Cell Signal. 2006 Oct;18(10):1758-68. Ep

   Insert

   G protein subunit Gamma 5 (Gng5 Rat)
   

   Use

   Tags

   His and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

8. HpMscS

   Plasmid

   #78310

   Purpose

   H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

   Depositor

   Douglas Rees

   Article

   Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

   Insert

   Mechanosensitive channel of small conductance
   

   Use

   Tags

   6x His Tag and FLAG Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCellFree_G03 NFKB1

   Plasmid

   #67126

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   NFKB1 (NFKB1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET15b-At 14-3-3 epsilon

    Plasmid

    #44936

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 epsilon (GRF10 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. H6-epsin 1 (144-575)

    Plasmid

    #63008

    Purpose

    expresses the adaptor protein epsin that lacks its ENTH-domain in bacteria

    Depositor

    Ernst Ungewickell

    Article

    Kalthoff et al J Biol Chem. 2002 Mar 8;277(10):8209-16.

    Insert

    Epsin 1 without ENTH domain (Epn1 Rat)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MEIS1A-pET

    Plasmid

    #21015

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

    Insert

    MEIS1A (Meis1 Mouse)
    

    Use

    Tags

    HIS and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCellFree_G03 DDX5

    Plasmid

    #67111

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. BacPAK Flag-NFRKB

    Plasmid

    #19791

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    NFRKB (NFRKB Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3_AKAR5Δ

    Plasmid

    #119912

    Purpose

    Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    AKAR5Δ
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQTEV-SARA1

    Plasmid

    #34809

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    SARA1 (SAR1A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBICI_2_strong

    Plasmid

    #155168

    Purpose

    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-EAF pBacPAK8

    Plasmid

    #15323

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    ELL Associated Factor (EAF1 Human)
    

    Use

    Baculovirus construction

    Tags

    6x His and FLAG

    Expression

    Mutation

    Promoter

    polyhedrin

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. spa-GCaMP6f

    Plasmid

    #67558

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    CMV

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pp32ΔCT

    Plasmid

    #67241

    Purpose

    pp32(1-149) cloned into pProEX HTb

    Depositor

    Cynthia Wolberger

    Article

    Huyton et al Protein Sci. 2007 Jul;16(7):1308-15. Epu

    Insert

    pp32 (ANP32A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-149

    Promoter

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

22. PM18033-PLS-KP6_rpoA-C

    Plasmid

    #190986

    Purpose

    Test the C-to-U editing activity of KP6 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PM18033-PLS-KP2_rpoA-U

    Plasmid

    #190976

    Purpose

    Test the U-to-C editing activity of KP2 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PM18033-PLS-KP3_rpoA-U

    Plasmid

    #190980

    Purpose

    Test the U-to-C editing activity of KP3 on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Prb

    Plasmid

    #24785

    Depositor

    David Baker

    Article

    Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

    Insert

    Prb
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pET-PLS-KP2_rpoA-U

    Plasmid

    #190956

    Purpose

    Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-PLS-KP6-A66S_rpoA-U

    Plasmid

    #190969

    Purpose

    Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:A66S

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-PLS-KP3_rpoA-U

    Plasmid

    #190960

    Purpose

    Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-PLS-KP6-E67A_rpoA-U

    Plasmid

    #190973

    Purpose

    Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    DYW-KP:E67A

    Promoter

    T7

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRSET-triple mutation-pa-GCaMP6f

    Plasmid

    #67570

    Purpose

    bacterial expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    pa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    V115H, L64F, T65S

    Promoter

    T7

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pET32b-FLJ23445

    Plasmid

    #15434

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    FLJ23445
    

    Use

    Tags

    His and Trx

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

32. Prb(Asp83Asn)

    Plasmid

    #24833

    Depositor

    David Baker

    Article

    Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

    Insert

    Prb(Asp83Asn)
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Asp83Asn

    Promoter

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

33. NK-1 GFP

    Plasmid

    #24508

    Depositor

    Ron Vale

    Article

    Case et al Cell. 1997 Sep 5. 90(5):959-66.

    Insert

    Drosophila NCD, human kinesin
    

    Use

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    NK-1 is a chimera where the entire superfamily-co…

    Promoter

    Available Since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

34. CKAP2 pHAT

    Plasmid

    #175946

    Purpose

    Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

    Depositor

    Susanne Bechstedt

    Article

    McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

    Insert

    CKAP2 (Ckap2 Mouse)
    

    Use

    Tags

    6-His and StrepTagII

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEBTetD-SNAP-Centrin3

    Plasmid

    #136807

    Purpose

    Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrin3
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available Since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. YFP Gamma 7

    Plasmid

    #36105

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 7 (GNG7 Bovine)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    HIs tag between YFP and insert

    Promoter

    CMV

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

37. pCellFree_G03 FYN

    Plasmid

    #67106

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FYN (FYN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pmCellFree_KA2_ZIKV NS2A

    Plasmid

    #145363

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2A
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJK2067

    Plasmid

    #171845

    Purpose

    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)

    Depositor

    Judith Kimble

    Article

    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:

    Insert

    his-58, eGFP, mCherry
    

    Use

    Tags

    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…

    Expression

    Worm

    Mutation

    boxB wild type and hairpin mutant

    Promoter

    mex-5 promoter

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCellFree_G03 CREBZF

    Plasmid

    #67070

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CREBZF (CREBZF Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only