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We narrowed to 66,118 results for: nin

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Showing: 5001 - 5020 of 66118 results

  1. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    114073-plasmid-map-sequence-id-220529

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  5. pJZC48

    Plasmid
    #62336
    Purpose
    sgRNA + 1x COM with COM-VP64 effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Publication
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 1x COM binding module
    COM-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA
    Promoter
    CMV and U6
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62336-plasmid-map-sequence-id-102259

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  8. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid
    #208048
    Purpose
    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    BirAopt
    Use
    Lentiviral
    Mutation
    BirAopt is human-codon-optimized BirA (PMID 15707…
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    208048-plasmid-map-sequence-id-410202

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  9. pGD-γb

    Plasmid
    #196327
    Purpose
    35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressor
    Depositor
    Zhenghe Li
    Publication
    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:
    Insert
    barley stripe mosaic virus γb
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196327-plasmid-map-sequence-id-390033

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  11. QUIN

    Plasmid
    #184267
    Purpose
    A binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.
    Depositor
    Feng Qu
    Publication
    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do
    Insert
    CPSMV RNA1 cDNA with 4 introns
    Expression
    Plant
    Mutation
    S1098P, K1651R
    Promoter
    CaMV 35S with duplicated enhancer
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184267-plasmid-map-sequence-id-363074

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  12. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    175570-plasmid-map-sequence-id-344992

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  15. pLenti-xBE4Gam-P2A-Puro

    Plasmid
    #112676
    Purpose
    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)
    Depositor
    Lukas Dow
    Publication
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Insert
    xCas9(3.7)-BE4Gam
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…
    Promoter
    EF1s
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112676-plasmid-map-sequence-id-217196

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  17. p240 pPr-CREM/Ins

    Plasmid
    #8405
    Purpose
    Androgen-inducible expression of Cre, with chimeric/β-globin intron within the Cre coding sequence and chicken globin insulators.
    Depositor
    Jeffrey Green
    Publication
    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56
    Insert
    probasin driven CREM with insulators
    Use
    Cre/Lox
    Expression
    Mammalian
    Mutation
    modified Cre
    Promoter
    Probasin promoter
    Available since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  18. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid
    #123235
    Purpose
    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    123235-plasmid-map-sequence-id-378779

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Showing: 5001 - 5020 of 66118 results