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pRK011

Plasmid

#250137

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(IDR) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK036

Plasmid

#250138

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pNMSB88

Plasmid

#199321

Purpose

Combine with Gal4 to direct TeNL expression

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::TeNL::let-858 3'UTR

Expression

Worm

Mutation

KD for calcium is 250 nM, 1 synthetic intron

Promoter

15xUAS::delta pes-10

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB109

Plasmid

#199322

Purpose

Combine with Gal4 to direct CaMBI 300 expression

Depositor

Michael Krieg

Article

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::CaMBI::let-858 3'UTR

Expression

Worm

Mutation

Orange CaMBI with 300 nM KD for calcium

Promoter

15xUAS:::delta pes-10

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-V5-SPR

Plasmid

#127236

Purpose

Eukaryotic expression of SPR

Depositor

Kai Johnsson

Article

Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

Insert

SPR (SPR Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::mOrange

Plasmid

#124346

Purpose

Nuclear Localized Pan-Neuronal mOrange

Depositor

Andrew Leifer

Insert

rab-3, mOrange (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZC_CeTRIC-B2_Del

Plasmid

#87345

Purpose

Inducible expression of CeTRIC-B2 in P.Pichia strains

Depositor

Zhenfeng Liu

Article

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B2

Tags

polyhistidine

Expression

Yeast

Mutation

Δ253-313

Promoter

AOX1

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pAAP35

Plasmid

#250142

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP36

Plasmid

#250143

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP37

Plasmid

#250144

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D2-IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK042

Plasmid

#250139

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-IDR) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK082

Plasmid

#250140

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Article

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-RGG) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC pdzd-8

Plasmid

#227844

Purpose

Used for transfection of cells for expression of C-MYC pdzd-8

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

pdzd-8 (C53B4.4 Nematode)

Tags

MYC

Expression

Mammalian

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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RT-5

Plasmid

#220442

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (puf-3 Nematode)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT239

Plasmid

#204522

Purpose

In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

Mos1_transposase::glh-2_3'UTR (glh-2 Nematode)

Use

Other

Promoter

T7 (minimum seq.)

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pdestMB14_grh-1p-grh-1-gfp

Plasmid

#177761

Purpose

Overexpression of C. elegans grh-1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

grh-1 (grh-1 Nematode)

Expression

Worm

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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TU#1412_tiam-1_cDNA

Plasmid

#82364

Purpose

express tiam-1 cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

tiam-1 cDNA (tiam-1 Nematode)

Expression

Worm

Promoter

none

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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