We narrowed to 40,933 results for: gats

1. pENTR-L3-Csy4-Cas9n-EGFP-L2

   Plasmid

   #141046

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-Cas9n-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGolden-Hyg

   Plasmid

   #51423

   Purpose

   Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

   Depositor

   Yonglun Luo

   Article

   Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

   Insert

   PGK-Hyg-pA (PGK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-APEX2-NS

   Plasmid

   #67669

   Purpose

   Gateway entry clone containing APEX2 with no stop codon

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   APEX2
   

   Use

   Gateway multisite middle entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. p667-UBC-CDC42BPB-V5-miniTurbo_IDG-K

   Plasmid

   #198008

   Purpose

   Gateway destination clone of CDC42BPB (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDC42BPB-V5-miniTurbo (CDC42BPB Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-miniTurbo-V5-CAMKK2_IDG-K

   Plasmid

   #170577

   Purpose

   Gateway destination clone of CAMKK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CAMKK2 (CAMKK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-CDK17_IDG-K

   Plasmid

   #158174

   Purpose

   Gateway destination clone of CDK17 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK17 (CDK17 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV/MASC_(GLuc)_TOP1

   Plasmid

   #68439

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. IF101: pMVP (L1-L4) EF1a promoter

   Plasmid

   #121687

   Purpose

   pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   EF1a promoter
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p667-UBC-BRSK2-V5-TurboID_IDG-K

   Plasmid

   #135253

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   BRSK2-V5-TurboID (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p667-UBC-CDK12-V5-miniTurbo_IDG-K

    Plasmid

    #135243

    Purpose

    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK12-V5-miniTurbo (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR223-GFP

    Plasmid

    #198589

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    GFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. FUW-mTurquoise

    Plasmid

    #193092

    Purpose

    constitutive expression of mTurquoise in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    mTurquoise
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-miniTurbo-V5-DYRK1B_IDG-K

    Plasmid

    #158183

    Purpose

    Gateway destination clone of DYRK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-DYRK1B (DYRK1B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HB401: pMVP (L1-L4) CMV promoter

    Plasmid

    #121686

    Purpose

    pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    CMV promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPS0314

    Plasmid

    #115507

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    celB pOGG050, T-pharma pOGG003 assembled in pOGG026
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUBC_HA_2xPP7-VP64

    Plasmid

    #68418

    Purpose

    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    PP7(deltaFG)
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    PP7: [delta]67_74 (non-aggregating)

    Promoter

    human UbC

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-miniTurbo-V5-CDK18_IDG-K

    Plasmid

    #158180

    Purpose

    Gateway destination clone of CDK18 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK18 (CDK18 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMVS-V1

    Plasmid

    #183094

    Purpose

    Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

    Depositor

    Bastian Molitor, Lars Angenent

    Article

    Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

    Inserts

    pME2001
    thermostable neomycin phosphotransferase gene
    

    Use

    Bacterial propagation and conjugative

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCVpuroATT

    Plasmid

    #47539

    Purpose

    Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

    Depositor

    David Weinstock

    Article

    Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid

    #68437

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U1

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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