We narrowed to 18,211 results for: puro

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pgRNA_hMECP2_promoter_No6

Plasmid

#194900

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-6 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.6

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pgRNA_hMECP2_promoter_No7

Plasmid

#194901

Purpose

Lentiviral construct with mCherry and Puro cassettes to express sgRNA-7 targeting the promoter of human MECP2.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Insert

sgRNA targeting human MECP2 promoter No.7

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Immune checkpoint (library 2) Backbone

Plasmid

#192363

Purpose

Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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T cell activation (library 1) Backbone

Plasmid

#192364

Purpose

Plasmid vector for in vitro Jurkat activation screening (for immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Pigb-V5

Plasmid

#175160

Purpose

Lentiviral expression of mouse Pigb-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Pigb (Pigb Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-Tmem209

Plasmid

#175154

Purpose

Lentiviral expression of V5-tagged mouse Tmem209

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem209 (Tmem209 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Zdhhc6-V5

Plasmid

#175146

Purpose

Lentiviral expression of mouse Zdhhc6-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Zdhhc6 (Zdhhc6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shSpc25

Plasmid

#160960

Purpose

Spc25 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Spc25 shRNA (Spc25 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shNuf2

Plasmid

#160961

Purpose

Nuf2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nuf2 shRNA (Nuf2 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgYAP-10

Plasmid

#121424

Purpose

sgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgYAP-10 (GTGCTGTCCCAGATGAACGTC)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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p-miR-SC

Plasmid

#65055

Purpose

Expression of miR148a with scrambled seed in mammalian cells

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

hsa-miR-148a (mutated) (MIR148A Human)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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T7-VEE-GFP

Plasmid

#58977

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLIX403_Capture1_APOBEC_HA_P2A_mRuby

Plasmid

#183901

Purpose

Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

Capture1_APOBEC_HA_P2A_mRuby

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMIA10.53-Clover

Plasmid

#214450

Purpose

BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

mClover

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-cFos-mCh

Plasmid

#193066

Purpose

constitutive expression of mouse cFos fused to mCherry in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

cfos-mCh

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide_DKO

Plasmid

#183193

Purpose

Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

Depositor

Lang Li

Article

Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6, mU6

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-STAT2

Plasmid

#71451

Purpose

3rd generation lentiviral expression of STAT2

Depositor

George Stark

Article

Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

Insert

STAT2 (STAT2 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS C396/7A-HA

Plasmid

#130917

Purpose

Expresses human cGAS-HA with C396/397A point mutations; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS C396/7A

Tags

HA

Expression

Mammalian

Mutation

mutated residues 396 and 397 to alanines

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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