We narrowed to 9,203 results for: sacs

1. pAM90

   Plasmid

   #226682

   Purpose

   Rpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)

   Depositor

   Andreas Martin

   Article

   Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

   Inserts

   Rpn5 (RPN5 Budding Yeast)
   Rpn6 (RPN6 Budding Yeast)
   Rpn8 (RPN8 Budding Yeast)
   Rpn9 (RPN9 Budding Yeast)
   Rpn11 (RPN11 Budding Yeast)
   

   Use

   Tags

   MBP-HRV

   Expression

   Bacterial

   Mutation

   S111TAG

   Promoter

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

   Plasmid

   #169135

   Purpose

   E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Coh7
   

   Use

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1:mTRESK(S116C)

   Plasmid

   #224772

   Purpose

   In vitro transcription for Oocyte expression of mTRESK(S116C)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily member 18 (Kcnk18 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S116C

   Promoter

   T7

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE:hTRAAK(S118C/Q258K)

   Plasmid

   #224764

   Purpose

   In vitro transcription for Oocyte expression of hTRAAK(S118C/Q258K)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily member 4 (KCNK4 Human)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S118C/Q258K

   Promoter

   T7

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES2-eGFP:HA-mTREK-1(S131C)

   Plasmid

   #224774

   Purpose

   Mammalian expression vector for generating mouse TREK-1

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S131C

   Promoter

   CMV

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMHE:mTREK-1(S131C)

   Plasmid

   #224744

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(S131C)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C

   Promoter

   T7

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEMHE:mTREK-1 (C159A/C219A)

   Plasmid

   #224751

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   C159A/C219A

   Promoter

   T7

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE:mTRAAK(S93C/Q233K)

   Plasmid

   #224768

   Purpose

   In vitro transcription for Oocyte expression of mTRAAK(S93C/Q233K)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily member 4 (Kcnk4 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S93C / Q233K

   Promoter

   T7

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEMHE:mTREK-1(S131A)

   Plasmid

   #224745

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(S131A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131A

   Promoter

   T7

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEMHE:mTRAAK(Q233K)

    Plasmid

    #224767

    Purpose

    In vitro transcription for Oocyte expression of mTRAAK(Q233K)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 4 (Kcnk4 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Q233K

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 TREK-2(S156C)

    Plasmid

    #224770

    Purpose

    In vitro transcription for Oocyte expression of 1 TREK-2(S156C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 10 (Kcnk10 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S156C

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEMHE:mTREK-1(G171F)

    Plasmid

    #224747

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(G171F)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    G171F

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEMHE:mTRAAK(S93C)

    Plasmid

    #224766

    Purpose

    In vitro transcription for Oocyte expression of mTRAAK(S93C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 4 (Kcnk4 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S93C

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEMHE:mTREK-1(S131C/K271Q)

    Plasmid

    #224750

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C / K271Q

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEMHE:mTREK-1(A286F)

    Plasmid

    #224746

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(A286F)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    A286F

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEMHE:mTREK-1(tandem wt-S131C)

    Plasmid

    #224759

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Tandem TREK-1 (wt-S131C) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S562C

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEMHE:mTREK-1(S131C/G171F)

    Plasmid

    #224749

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(S131C/G171F)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C / G171F

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEMHE:mTREK-1(tandem S131C-wt)

    Plasmid

    #224758

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEMHE:mTREK-1(S131C/A286F)

    Plasmid

    #224748

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C / A286F

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-C1-FKBPflex-ELL-CAAX

    Plasmid

    #220076

    Purpose

    an extra long linker attached to a CAAX domain with an mCherry tag

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    CAAX (HRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pToxamEXP4CUP1

    Plasmid

    #218284

    Purpose

    Use together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    Hph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pIRGAL43C

    Plasmid

    #218278

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GAL3 (S509P)

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pIRGAL4A

    Plasmid

    #218277

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIRGAL3C

    Plasmid

    #218275

    Purpose

    Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GAL3 (S509P)

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YIp128-CUP1-His6-CUbo(K48R)-GFP

    Plasmid

    #212793

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His6-CUbo(K48R)-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    CUb(K48R)

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. YIp128-CUP1-His6-CUbo(K63R)-GFP

    Plasmid

    #212794

    Purpose

    Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    His6-CUbo(K63R)-GFP
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    CUb(K63R)

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YIp211-ADH-NUbo-E3(48) aa24-150-VSV

    Plasmid

    #212747

    Purpose

    Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48) aa24-150
    

    Use

    Integrative vector

    Tags

    NUbo and VSV

    Expression

    Yeast

    Mutation

    Cue1 ΔU7BD

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

    Plasmid

    #212738

    Purpose

    Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-NLS-VSV and myc

    Expression

    Yeast

    Mutation

    HOIP(C885A)

    Promoter

    ADH1

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

    Plasmid

    #202090

    Purpose

    Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

    Depositor

    Elizabeth Heller

    Article

    Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

    Insert

    dCas9-Set2(R195G, C201A)
    

    Use

    Tags

    3x FLAG and mCherry

    Expression

    Mammalian

    Mutation

    R195G and C201A abolish catalytic activity

    Promoter

    human Synapsin

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

    Plasmid

    #212805

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge