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J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1980

Plasmid

#49111

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-iCre

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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SIN-PGK-Cas9-V5-WPRE

Plasmid

#87876

Purpose

To produce lentiviral vector for Cas9 editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

spCas9

Use

Lentiviral

Tags

V5

Mutation

Human codon-optimized

Promoter

mouse PGK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-GFP

Plasmid

#82506

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR

Plasmid

#75136

Purpose

Retroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EHD2b

Plasmid

#109517

Purpose

Muscle specific zebrafish expression of mKate2 fused to EHD2b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD2b (ehd2 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2166

Plasmid

#158412

Purpose

High Copy number plasmid containing Ple331 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple331 (PAX6 Human)

Use

Unspecified

Promoter

Ple331

Available Since

Feb. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR

Plasmid

#75135

Purpose

Retroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A

Plasmid

#32456

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S129A, S133A, S137A (S3V, G96R, and P108L also pr…

Promoter

SV40

Available Since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pEMS2158

Plasmid

#70119

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP

Use

AAV

Expression

Mammalian

Promoter

Promoterless

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1993

Plasmid

#49123

Purpose

AAV plasmid with OLIG1 (Ple304) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple304-iCre

Use

AAV

Expression

Mammalian

Promoter

OLIG1

Available Since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2275

Plasmid

#111893

Purpose

High Copy number plasmid containing Ple345 (NEFL MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple345 (NEFL Human)

Use

Unspecified

Promoter

Ple345 (NEFL MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCRII-Blunt-U6-HES7-STOP-gRNA

Plasmid

#130933

Purpose

gRNA for tag human HES7

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7-STOP-gRNA (HES7 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

Plasmid

#192471

Purpose

Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12

Tags

AcGFP1

Expression

Insect

Promoter

Ac5

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-0-YFP

Plasmid

#41663

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; C-terminal hyd…

Promoter

CMV

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1YFP20

Plasmid

#41671

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 20 C-terminal-…

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1CFP8

Plasmid

#41670

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; 8 C-terminal-m…

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP4

Plasmid

#129502

Purpose

Expresses AausFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP4

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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FusXTBE-1397-C

Plasmid

#201398

Purpose

Expresses C terminus half of DddAtox in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-LacZ-G1397 DddAtox-C-UGI-ATP5B 3'UTR

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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FusXTBE-1397-N

Plasmid

#201397

Purpose

Expresses N terminus half of DddAtox in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-LacZ-G1397 DddAtox-N-UGI-ATP5B 3'UTR

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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