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pmMBD2∆CC-G pc5088

Plasmid

#229764

Purpose

Mammalian expression plasmid of MBD2∆CC. Protein is lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Protein is lacking the coiled coil domain: aa 363…

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2∆N∆CC-G pc5089

Plasmid

#229765

Purpose

Mammalian expression plasmid of MBD2∆N∆CC. Protein is lacking the coiled coil domain: aa 363-396 and the N-terminus: aa 1-152. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2.2-GFP pc2068

Plasmid

#229560

Purpose

Mammalian expression vector codes for MBD2(aa 153-414) fused c-terminal to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

MBD2(aa 153-414)

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmApple-EGFP-HRas

Plasmid

#133854

Purpose

Mammalian expression of HRas N-terminally tagged with mApple and EGFP

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

HRas (HRAS Human)

Tags

EGFP and mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP(1-233)-mApple(2-236)-HRas(2-189)

Plasmid

#133855

Purpose

Mammalian expression of HRas N-terminally tagged with EGFP and mApple

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

HRas (HRAS Human)

Tags

EGFP and mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mEos2-Farnesyl-5

Plasmid

#57375

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Catherine Galbraith

Article

Jaqaman et al Mol Biol Cell. 2016 May 15;27(10):1561-9

Insert

Farnesyl (HRAS Human)

Tags

mEos2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pKT0176

Plasmid

#8743

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

tdimer2

Tags

Codon optimized linker

Expression

Yeast

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/Halo-CAAX

Plasmid

#249173

Purpose

Mammalian expression vector of CAAX motif (plasma membrane marker) fused with Halo-tag

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

CAAX (HRAS Human)

Tags

Halo

Expression

Mammalian

Mutation

Only 10 amino acids ending with CVLS (CAAX motif)

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-GJA1-20k-N1

Plasmid

#49861

Purpose

Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

GJA1-20k (GJA1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

GJA1-20k corresponds to residues 213 to 382 to Co…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2

Plasmid

#172115

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSL1423

Plasmid

#194904

Purpose

pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparum

Depositor

Scott Lindner

Insert

TurboID

Use

Plasmodium falciparum

Tags

GFPmut2

Promoter

None. This allows a C-terminal fusion to a protei…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_NLuc_FRT-PuroR

Plasmid

#177865

Purpose

Cloning Backbone for NanoLuc-luciferase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

NLuc-based EXSISERS

Use

CRISPR, Synthetic Biology, and Unspecified

Tags

OLLAS

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_BSD_FRT-PuroR

Plasmid

#177867

Purpose

Cloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

BSD-based EXSISERS

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

OLLAS

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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loxP-G418-loxP-3xALFA-Halo-Rab11 HR

Plasmid

#229676

Purpose

Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence Rab11 locus (RAB11A Human)

Use

CRISPR

Tags

Halo, 3xALFA

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF2-FL-hMer_gR1

Plasmid

#225993

Purpose

Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor

Depositor

Raymond Birge, Sergei Kotenko

Article

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLENTI-PGK-HYGRO-GATA6mut-HA

Plasmid

#65476

Purpose

Lentiviral expression of GATA6 Znc-finger mutant

Depositor

Eduard Batlle

Article

Whissell et al Nat Cell Biol. 2014 Jul;16(7):695-707. d

Insert

GATA6mut (GATA6 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

lacking the eight most highly conserved bases of …

Promoter

PGK

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pEF2-EFGR-hAxl

Plasmid

#225997

Purpose

Expresses a chimeric contruct having N-terminal human EGFR and C-terminal human Axl

Depositor

Raymond Birge, Sergei Kotenko

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human Axl C-Terminal Fusion (EGFR, AXL Human)

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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CXCR4-SZ158a-sfGFP

Plasmid

#162446

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-X-C chemokine receptor type 4 (CXCR4 Human)

Tags

sfGFP

Expression

Mammalian

Mutation

truncation from aa52 to aa257

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pHygG6

Plasmid

#200219

Purpose

hygR selection GFP marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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