We narrowed to 9,377 results for: tre promoter

1. pDAC439

   Plasmid

   #195237

   Purpose

   Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAC565

   Plasmid

   #195242

   Purpose

   Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a(+)-T7-OmpA-MNaseA-6xHis

   Plasmid

   #214808

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease A
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a(+)-T7-OmpA-MNaseB-6xHis

   Plasmid

   #214809

   Purpose

   Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

   Depositor

   Peter Sarin

   Article

   Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

   Insert

   OmpA-Micrococcal nuclease B
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDAC569

   Plasmid

   #195241

   Purpose

   Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICSL1P1_AtU626Ter26

   Plasmid

   #191769

   Purpose

   Cas9 guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   SpCas9 guide scaffold with AtU626 promoter/terminator
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4/TO-cMYC

   Plasmid

   #216510

   Purpose

   Expresses MYC in human cells under a tetracycline/doxycycline-inducible promoter

   Depositor

   John Diffley

   Article

   Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

   Insert

   cMYC
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKM197

   Plasmid

   #132665

   Purpose

   pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

   Depositor

   Joseph Sorg

   Article

   Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

   Insert

   Upstream & Downstream pyrE deletion region
   

   Use

   E. coli - c. difficile shuttle vector

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSfi-HspLacZ

    Plasmid

    #33351

    Depositor

    David Kingsley, Doug Mortlock

    Article

    Mortlock et al Genome Res. 2003 Sep;13(9):2069-81. Epub

    Insert

    Hsp68 promoter (Hspa1a Mouse)
    

    Use

    Mouse Targeting

    Tags

    LacZ

    Expression

    Mammalian

    Promoter

    Hsp68 (Hspa1a)

    Available Since

    Jan. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pAGM53151

    Plasmid

    #169093

    Purpose

    Level 1 cloning vector for transient expression. Already contains the 35S promoter and the Nos terminator.

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

    Type

    Empty backbone

    Use

    Moclo cloning vector

    Expression

    Plant

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSuper-PICK1 shRNA

    Plasmid

    #67522

    Purpose

    Expresses shRNA against PICK1

    Depositor

    Victor Anggono, Richard Huganir

    Article

    Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

    Insert

    PICK1 (Pick1, Pick1 Mouse, Rat)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1 Promoter

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICSL1P3_AtU626Ter26

    Plasmid

    #191771

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pG1-NMGR526

    Plasmid

    #1122

    Depositor

    Susan Lindquist

    Article

    Li et al Science 2000 Jan 28;287(5453):661-4.

    Insert

    sup35 NM domain fused with GR (SUP35, Nr3c1 Budding Yeast, Rat)
    

    Tags

    GR526

    Expression

    Yeast

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pICSL1P4_AtU626Ter26

    Plasmid

    #191772

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICSL1P7_AtU626Ter26

    Plasmid

    #191775

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICSL1P2_AtU626Ter26

    Plasmid

    #191770

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICSL1P5_AtU626Ter26

    Plasmid

    #191773

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICSL1P6_AtU626Ter26

    Plasmid

    #191774

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Avs4_Chimera

    Plasmid

    #188802

    Purpose

    Chimeric Avs4 with transmembrane effector

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Avs4 chimera
    

    Expression

    Bacterial

    Promoter

    EcAvs4 native promoter

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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