We narrowed to 3,156 results for: FRI

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EGFPC2-Gephyrin P1

Plasmid

#68815

Purpose

expression of fluorescent tagged Geph in mammalian cells

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyri…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C14A

Plasmid

#89977

Purpose

Expresses catalytic inactive GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 14 to Alanine

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABE8e_dNRCH-BlastR

Plasmid

#232720

Purpose

A-to-G base editor

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

ABE8e-dCas9(NRCH)

Expression

Mammalian

Mutation

within Cas9 D10A H840A

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-NLS-actin-R62D

Plasmid

#118381

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS and R62D

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA as2

Plasmid

#132394

Purpose

Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

Plasmid

#220493

Purpose

To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)

Use

CRISPR

Promoter

U6

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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yeast_DualReporter

Plasmid

#127546

Purpose

A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).

Depositor

Aviv Regev

Article

de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do

Inserts

bla
mCherry
URA3 (URA3 Budding Yeast)
yEVenus
Nat1

Use

Synthetic Biology

Expression

Yeast

Promoter

TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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GPX8 rescue PLAK1

Plasmid

#161517

Purpose

Rescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells

Depositor

Yoav Shaul

Article

Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

Insert

glutathione peroxidase 8 (GPX8 Human)

Use

Lentiviral

Tags

3X Flag

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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