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pGL0_17 [AMA1 short ORI]

Plasmid

#198930

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

AMA1 short ORI

Use

Synthetic Biology

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_177

Plasmid

#199109

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_174

Plasmid

#199106

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pNG2 ORI plus NGS barcode 22, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_165

Plasmid

#199097

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSC101ts ORI plus NGS barcode 10, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_161

Plasmid

#199093

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAL5000 ORI plus NGS barcode 1, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_162

Plasmid

#199094

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pUB110 ORI plus NGS barcode 2, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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vgCam-pRSETb

Plasmid

#193362

Purpose

A FRET-based calcium probe using Sumire

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

vgCam

Use

E.coli expression

Tags

His X6 tag and Strep tag

Promoter

T7

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pT2MPEF-TOMM-QUE37C

Plasmid

#191526

Purpose

ATP measurement, mitochondria outer membrane, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81D

Plasmid

#129351

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81D

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu E83A

Plasmid

#129353

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu E83A

Expression

Bacterial

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLBS.EF-H2B-QUE37C

Plasmid

#129327

Purpose

ATP measurement, nucleus, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pPPC020.306

Plasmid

#171148

Purpose

Expression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J306 scRNA
J3-BBa_J23117-mRFP

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J3-BBa_J23117

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBEAST_J23101-amidase

Plasmid

#128135

Purpose

The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

amidase

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003-sgPOLR2D

Plasmid

#125769

Purpose

constitutive expression of a guide RNA targeting human POLR2D (CRISPR positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgPOLR2D (POLR2D Human)

Use

CRISPR

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCR4_SNORD27 probe

Plasmid

#73069

Purpose

antisense probe for detection of human SNORD27

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (SNORD2 Human)

Use

In vitro transcription

Promoter

t7

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCarCBADE

Plasmid

#117757

Purpose

Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised CarCBA, Protein CarD, Ferredoxin CarE

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314-PRP16

Plasmid

#89971

Purpose

Prp16 in a trp backbone complements prp16 genomic deletion

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

none

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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PHBA02

Plasmid

#83536

Purpose

Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

Depositor

Claudia Vickers

Article

Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

Insert

pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFBXC3H

Plasmid

#49050

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFBXNH3

Plasmid

#49049

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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