We narrowed to 11,278 results for: VARS

1. pCAT526

   Plasmid

   #180513

   Purpose

   Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv1
   PlmCasX-2A-mNeonGreen
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HEL binding scFv D44.1

   Plasmid

   #111718

   Purpose

   Yeast Surface Display of D44.1 as a reference starting point for design

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   D44.1
   

   Tags

   cmyc tag

   Expression

   Yeast

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe EGFR Del5(T790M)

   Plasmid

   #32069

   Depositor

   Matthew Meyerson

   Article

   Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

   Insert

   EGFR (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Delete E746-S752, insert V, T790M

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

4. p1.2-GS

   Plasmid

   #162770

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-K1LM04

   Plasmid

   #103119

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

   Plasmid

   #185328

   Purpose

   For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   MAHS_RAMVA_81-236-FLAG
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDual-eGFP(W66)

   Plasmid

   #63217

   Purpose

   Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(W66)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pEMS1989

   Plasmid

   #49120

   Purpose

   AAV plasmid with CAGGS promoter driving expression of iCre. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-CAGGS-iCre-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. sgRNA BRCA2 R2842C

   Plasmid

   #139324

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 R2842C using base editing
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS2177

    Plasmid

    #111892

    Purpose

    High copy number plasmid containing Ple344 (TUBB3 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple344 (TUBB3 Human)
    

    Use

    Unspecified

    Promoter

    Ple344 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEMS2175

    Plasmid

    #111890

    Purpose

    High copy number plasmid containing Ple342 (TUBB3 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple342 (TUBB3 Human)
    

    Use

    Unspecified

    Promoter

    Ple342 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEMS2176

    Plasmid

    #111891

    Purpose

    High copy number plasmid containing Ple343 (TUBB3 MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple343 (TUBB3 Human)
    

    Use

    Unspecified

    Promoter

    Ple343 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pUC19-E6I4Q5

    Plasmid

    #103101

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1P MUTYH_2

    Plasmid

    #160786

    Purpose

    Suppress MUTYH

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shMUTYH_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P MUTYH_1

    Plasmid

    #160785

    Purpose

    Suppress MUTYH

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shMUTYH_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEMS1984

    Plasmid

    #49115

    Purpose

    AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple253-iCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    PITX3

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pHH0103_CRKL-2/2

    Plasmid

    #91364

    Purpose

    Protein expression and purification of human SH3 domain construct CRKL-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRKL-2/2 (CRKL Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CRISPR V2 sgShmt2_1

    Plasmid

    #106314

    Purpose

    Express Cas9 and sgRNA targeting mShmt2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting SHMT2 (Shmt1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS2276

    Plasmid

    #111894

    Purpose

    High Copy number plasmid containing Ple346 (NEFM MiniPromoter) insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple346 (NEFM Human)
    

    Use

    Unspecified

    Promoter

    Ple346 (NEFM MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126777

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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