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Addgene

We narrowed to 5,680 results for: SUP

Showing: 5101 - 5120 of 5680 results
  1. pJBL-RNAINS4-C24A

    Plasmid
    #69462
    Purpose
    RNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Insert
    RNA-IN S4 with C24A mutation
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    C24A and mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pJBL-RNAINS4-A25C

    Plasmid
    #69461
    Purpose
    RNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Insert
    RNA-IN S4 with A25C mutation
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    A25C and mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pJBL-RNAINS4-C24AA25C

    Plasmid
    #69463
    Purpose
    RNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Insert
    RNA-IN S4 with C24A, A25C mutations
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    C24A, A25C, and mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
  5. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pET28a-HsSENP1

    Plasmid
    #71465
    Purpose
    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Insert
    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pAS1NB m Rosella I

    Plasmid
    #71247
    Purpose
    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.
    Depositor
    Insert
    Rosella
    Use
    Tags
    yeast mitochondrial targeting sequence of citrate…
    Expression
    Yeast
    Mutation
    Promoter
    PGK1
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    Insert
    mCherry-pHluorin
    Use
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Mutation
    Promoter
    pDAB
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 5101 - 5120 of 5680 results