We narrowed to 143,240 results for: ins

1. VPS13A^Halo

   Plasmid

   #118759

   Purpose

   VPS13A internally tagged with Halo at residue 1372.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13A (VPS13A Human)
   

   Use

   Tags

   Halo tag inserted at position 1372

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE-80L MBP-SspB Nano

   Plasmid

   #60409

   Purpose

   E. coli expression of SspB Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   SspB nano
   

   Use

   Tags

   His-MBP-TEV

   Expression

   Bacterial

   Mutation

   Y11K and A15E

   Promoter

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLL7.0: Venus-iLID-Mito (From ActA)

   Plasmid

   #60413

   Purpose

   Mammalian Expression of Venus-iLID-Mito

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   Venus-iLID-Mito
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. raACR-mCerulean3-pAAV2-hSyn

   Plasmid

   #204966

   Purpose

   Panneuronal expression of the MsACR1 mutant raACR fused to mCerulean3

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   raACR
   

   Use

   AAV

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Tn7 helper plasmid

   Plasmid

   #141161

   Purpose

   Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Insert

   Tn7 transposon machinery
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose-inducible pBAD

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 3xFlag IFIT1

   Plasmid

   #53554

   Purpose

   mammalian expression of IFIT1

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT1 (IFIT1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLL7.0: tgRFPt-SSPB R73Q

   Plasmid

   #60416

   Purpose

   Mammalian Expression of tgRFPt-Micro

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   tgRFPt-SSPB R73Q
   

   Use

   Cre/Lox and Lentiviral

   Tags

   TagRFPt

   Expression

   Mammalian

   Mutation

   R73Q, Y11K and A15E

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV500 A-FOS

   Plasmid

   #33353

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   FOS (Fos Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative of c-Jun; heterodimerizes and i…

   Promoter

   CMV

   Available since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-mNeptune2-N

   Plasmid

   #41645

   Purpose

   Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

   Insert

   mNeptune2-Dronpa145N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K145N on Dronpa (allows control of photochromic F…

   Promoter

   CMV

   Available since

   Dec. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJRoC30_VP8H

    Plasmid

    #188062

    Purpose

    PROSS-designed versatile peroxidase 8H

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

    Insert

    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    

    Use

    Tags

    S. cerevisiae native α-factor prepro-leader signa…

    Expression

    Yeast

    Mutation

    38 PROSS mutations, described in publication

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRII_TOPO_hNEAT1

    Plasmid

    #61518

    Purpose

    human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

    Depositor

    Archa Fox

    Article

    Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

    Insert

    NEAT1 (NEAT1 Human)
    

    Use

    General cloning vector

    Tags

    Expression

    Mutation

    *see comment below

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBABE puro human IRS-1

    Plasmid

    #11025

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 2Bc-T MBP_BoMoC(ed)_F753A_6xH

    Plasmid

    #185710

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed F753 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 3xFLAG TPP1 88-544

    Plasmid

    #53548

    Purpose

    mammalian expression of TPP1 full length

    Depositor

    Kathleen Collins

    Article

    Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSF-G1(7FTrp)RS

    Plasmid

    #177310

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDS2142

    Plasmid

    #184273

    Purpose

    Tet-off genome insertion system

    Depositor

    Dominique Sanglard

    Article

    Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

    Insert

    CatTA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDV24_AAV2_DART-VADAR_iRFP720-sensor

    Plasmid

    #200411

    Purpose

    AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    DART VADAR sensor
    

    Use

    AAV and Synthetic Biology

    Tags

    MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-X1-Neo-GFP-FAM134B-LIRmut

    Plasmid

    #109027

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    FAM134B-LIRmut (RETREG1 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

    Promoter

    EIF-1a promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL7.0: Venus-iLID-CAAX (from KRas4B)

    Plasmid

    #60411

    Purpose

    Mammalian Expression of Venus-iLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge