Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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pCMV-HA-ENO1-NU

Plasmid

#255450

Purpose

Mammalian expression of HA-tagged human ENO1

Depositor

Christine Mayr

Article

Luo et al Cell. 2026 Jun 8:S0092-8674(26)00576-3.

Insert

ENO1 (ENO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-ENO1-U

Plasmid

#255451

Purpose

HA-tagged human ENO1 with its own 3'UTR

Depositor

Christine Mayr

Article

Luo et al Cell. 2026 Jun 8:S0092-8674(26)00576-3.

Insert

ENO1 (ENO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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PHB1_BirA*

Plasmid

#251206

Purpose

Expresses human PHB1 tagged with BirA* in mammalian cells

Depositor

Svetlana Konovalova

Insert

Prohibitin 1 (PHB1 Human)

Use

Retroviral

Tags

BirA*

Expression

Mammalian

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-beta/alpha-14-3-3

Plasmid

#250062

Purpose

Bacterial expression of beta/alpha 14-3-3

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Beta/Alpha (YWHAB Human)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-8a)

Plasmid

#241069

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-8a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-8a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,L452R,F490R,Q493S,S494R,Q498Y,K529L,T…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-9a)

Plasmid

#241070

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-9a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-9a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S155R,R346T,L452R,F490R,Q493S,S494R,Q498Y,K…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-5a)

Plasmid

#241067

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-5a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-5a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147N,S155R,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-7a)

Plasmid

#241068

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-7a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-7a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,K147N,S155R,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-4a)

Plasmid

#241065

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,P25del,P26del,A27S,T76I,G142D,M153S,V213G,G3…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-4b)

Plasmid

#241066

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,T76I,G142D,M153S,V…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2g)

Plasmid

#241064

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2g) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2g) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S155R,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2e)

Plasmid

#241062

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2e) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2e) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S494R,K529L,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2f)

Plasmid

#241063

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2f) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2f) (S SARS-CoV-2)

Expression

Mammalian

Mutation

S494R,K147N,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2c)

Plasmid

#241060

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2d)

Plasmid

#241061

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

F490R,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2a)

Plasmid

#241058

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19I,L24del,P25del,P26del,A27S,G142D,V213G,G339D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDMJ2-SARS-CoV-2-Spike (BA.2.12.1-2h)

Plasmid

#241059

Purpose

Expresses SARS-CoV-2 spike (BA.2.12.1-2h) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (BA.2.12.1-2h) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,S494R,T19I,L24del,P25del,P26del,A27S,G142D,…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgSHMT2

Plasmid

#217435

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting SHMT2 (SHMT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 F456L (CTT)

Plasmid

#212542

Purpose

Encodes SARS-CoV-2 Spike F456L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike F456L (CTT) (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

F456L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-CC

Plasmid

#225476

Purpose

Express mEGFP-GAPDH-CC fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with CC2 domain of ROCK2 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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