We narrowed to 14,213 results for: cas9

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pBHM2674

Plasmid

#241727

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BSR marker for selection on blasticidin S

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2672

Plasmid

#241719

Purpose

Integration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains BLE marker for selection on phleomycin

Depositor

Hiten Madhani

Article

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pJAT22

Plasmid

#204311

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-GFPn

Use

CRISPR

Promoter

3XP3

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2ATetP2AP - mAGH

Plasmid

#229973

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2ATetP2AP - mAGH

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2ATetP2AP - H

Plasmid

#229972

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2ATetP2AP - H

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2AmcherryP2AP -TagBFPH

Plasmid

#229971

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2AmcherryP2AP -TagBFPH

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222857

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CTG)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCTG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222859

Purpose

This plasmid codes for the Slug-KH nickase with a sg(AGC)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgAGC

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222858

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CAG)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCAG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222860

Purpose

This plasmid codes for the Slug-KH nickase with a sg(GCA)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgGCA

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMLS713

Plasmid

#188885

Purpose

Multisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Multisite gateway [4-3] destination vector

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pOC5 cloning template vector

Plasmid

#183424

Purpose

FlpOFF knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOC3 cloning template vector

Plasmid

#183422

Purpose

CreON FlpOFF knock-in vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Type

Empty backbone

Use

CRISPR

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKEW395_AcrIIA14_truncated

Plasmid

#138284

Purpose

Expression of truncated AcrIIA14 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA14

Use

CRISPR

Tags

MBP

Expression

Bacterial

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW353_AcrIIA13_truncated

Plasmid

#138282

Purpose

Expression of truncated AcrIIA13 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA13

Use

CRISPR

Tags

MBP

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW398_AcrIIA15_truncated

Plasmid

#138286

Purpose

Expression of truncated AcrIIA15 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR

Tags

SFGFP

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAP757-1

Plasmid

#99493

Purpose

6XHis::TEV::3XFlag::linker::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

6XHis::TEV::3XFlag::linker::Myc

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP605-1

Plasmid

#99482

Purpose

Myc::TEV::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::TEV::Myc

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP686-1

Plasmid

#99491

Purpose

TEV::eGFP::ER::V5::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::V5::linker::3XFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP640-2

Plasmid

#99487

Purpose

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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