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pSMP-G9A_3

Plasmid

#36333

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP3(307-574)

Plasmid

#71481

Purpose

Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Deleted amino acids 1-306 (catalytic domain only)

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
pLib6.4-Aalb_744

Plasmid

#176663

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029744) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSR29

Plasmid

#69255

Purpose

pelt-2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

Promoter

elt-2

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD3_3

Plasmid

#36373

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD3 (MBD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pLib6.4-Cquin_596

Plasmid

#176655

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBI101.1/ProGOXL1:GUS

Plasmid

#175557

Purpose

Binary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genes

Depositor

George Haughn

Article

Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

Insert

ProGOXL1

Tags

GUS

Expression

Plant

Promoter

GOXL1

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-HsSENP5

Plasmid

#71468

Purpose

Encodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 5 (SENP5 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pSMP-RING1_1

Plasmid

#36355

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

RING1 (RING1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pET23a-MmRPS27a

Plasmid

#69559

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Mouse, Human)

Expression

Bacterial

Mutation

Changed Alanine 133 (human RPS27a) to Glycine (mo…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pSMP-MBD2_2

Plasmid

#36369

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD2_1

Plasmid

#36368

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
secCitrine

Plasmid

#181964

Purpose

Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

signal peptide sequence from basic chitinase

Tags

Citrine YFP

Expression

Plant

Promoter

1.5 kb MUM4 promoter

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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M-ST1-VPR

Plasmid

#68498

Purpose

nuclease competent ST1-Cas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

ST-Cas9-VPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Cquin_596

Plasmid

#176669

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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gRD21A-mRFP

Plasmid

#181959

Purpose

RD21A protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

RD21A genomic sequence including 2kb upstream of the ATG

Tags

mRFP

Expression

Plant

Promoter

RD21A native

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_726

Plasmid

#176661

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029726) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4-Aaeg_763

Plasmid

#176658

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017763) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMP-MBD1_1

Plasmid

#36365

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD1 (MBD1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD1_2

Plasmid

#36366

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD1 (MBD1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only