We narrowed to 7,101 results for: tev

1. gZiPro W83F

   Plasmid

   #198446

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   glycine linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   R95*A, W83F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. gZiPro W69F

   Plasmid

   #198445

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   glycine linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   R95*A, W69F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. eZiPro

   Plasmid

   #198434

   Purpose

   Activity/inhibition assays, 19F-NMR

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. gZiPro W50F

   Plasmid

   #198444

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   glycine linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   R95*A, W50F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTln1-F2-pET151

   Plasmid

   #177870

   Purpose

   Expresses mouse Talin 1 F2 domain in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2 domain (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mTln1-F2F3-pET151

   Plasmid

   #177877

   Purpose

   Expresses mouse Talin 1 F2F3 domains in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2F3 domain (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJCC_162 SpCas9 KES(1107-1109)GG

   Plasmid

   #179526

   Purpose

   For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 KES(1107-1109)GG
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   replaced KES (residues 1107-1109) with GG

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

   Plasmid

   #179527

   Purpose

   For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 K233A/K234A/K253A/K263A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed lysines 233, 234, 253, and 263 to alanine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T3-GST-PolB(K206A/K244A)

   Plasmid

   #177135

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EcRfaHKOW-L1L2NusG

    Plasmid

    #113126

    Purpose

    Expresses C-terminus of E. coli RfaH with L1 and L2 loops replaced with NusG residues

    Depositor

    James Berger

    Article

    Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

    Insert

    RfaH (rfaH Escherichia coli)
    

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Bacterial

    Mutation

    Deleted residues 1-107, replaced L1 and L2 loops …

    Promoter

    T7

    Available since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF11-SFPQ(214-535)

    Plasmid

    #135439

    Purpose

    Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    splicing factor proline and glutamine rich (SFPQ Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNH-TrxT-S18

    Plasmid

    #127826

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDDGFP-Leu2d-GgKDELR2 R159K

    Plasmid

    #123626

    Purpose

    Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg159Lys

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDDGFP-Leu2d-GgKDELR2 H12A

    Plasmid

    #123619

    Purpose

    Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    His12Ala

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDDGFP-Leu2d-GgKDELR2 R47A

    Plasmid

    #123620

    Purpose

    Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg47Ala

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDDGFP-Leu2d-GgKDELR2 R47K

    Plasmid

    #123621

    Purpose

    Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg47Lys

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDDGFP-Leu2d-GgKDELR2 E127A

    Plasmid

    #123622

    Purpose

    Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Glu127Ala

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDDGFP-Leu2d-GgKDELR2 E127Q

    Plasmid

    #123623

    Purpose

    Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Glu127Gln

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDDGFP-Leu2d-GgKDELR2 Y158F

    Plasmid

    #123624

    Purpose

    Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Tyr158Phe

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDDGFP-Leu2d-GgKDELR2 R159A

    Plasmid

    #123625

    Purpose

    Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg159Ala

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge