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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,825 results for: 158

Showing: 7741 - 7770 of 9825 results

  5. CMV-mito-R-GECO1

    Plasmid
    #46021
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    R-GECO1
    Use
    Tags
    a duplex of the mitochondrial targeting signal of…
    Expression
    Mammalian
    Mutation
    Substitutions relative to the mApple-derived anal…
    Promoter
    CMV
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    46021-plasmid-map-sequence-id-350822

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  6. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  8. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid
    #208963
    Purpose
    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    EcKlenow-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208963-plasmid-map-sequence-id-415798

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  9. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

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  15. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid
    #167288
    Purpose
    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.
    Depositor
    Agnel Sfeir
    Article
    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:
    Insert
    DDX58 (RIGI Human)
    Use
    Lentiviral
    Tags
    APEX2 and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167288-plasmid-map-sequence-id-330940

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  18. ACBE-Q

    Plasmid
    #204608
    Purpose
    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204608-plasmid-map-sequence-id-404717

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  19. Artichoke

    Plasmid
    #73320
    Purpose
    Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)
    Depositor
    Benjamin Ebert
    Article
    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and EF1a
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    73320-plasmid-map-sequence-id-135028

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  24. pHes7-Achilles-Hes7

    Plasmid
    #153528
    Purpose
    Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.
    Depositor
    Ryoichiro Kageyama
    Article
    Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:
    Insert
    Hes7 (Hes7 Mouse)
    Use
    Tags
    Achilles
    Expression
    Mammalian
    Mutation
    Promoter
    mouse Hes7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153528-plasmid-map-sequence-id-305297

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Showing: 7741 - 7770 of 9825 results