We narrowed to 11,231 results for: SHA

1. pGL3-321/+120

   Plasmid

   #35540

   Depositor

   Greg Goodall

   Article

   Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

   Insert

   promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   none

   Available since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500

   Plasmid

   #92317

   Purpose

   Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3

   Depositor

   Lital Alfonta

   Article

   Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

   Insert

   Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
   

   Use

   Synthetic Biology; Cell free protein synthesis (c…

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   p70a

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MKV937/nlp-1p::FlincG3

   Plasmid

   #140508

   Purpose

   This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

   Depositor

   Miri VanHoven

   Article

   Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

   Insert

   FlincG3
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   nlp-1

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2 mCherry-Rtn4HD

   Plasmid

   #86685

   Purpose

   Lentivirus to stably express fluorescent human protein in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   Rtn4 homology domain (RTN4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   homology domain only

   Promoter

   CMV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSAM2_mCherry_Nkx2.5

   Plasmid

   #72689

   Purpose

   Doxycyline regulated expression of Nkx2.5 in mammalian cells

   Depositor

   Timothy Kamp

   Article

   Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

   Insert

   Nkx 2.5 (Nkx2-5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-Doxycycline

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-his-Gluc-iLID

   Plasmid

   #172096

   Purpose

   Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria

   Depositor

   Avi Schroeder

   Article

   Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

   Insert

   Gaussia Luciferase - iLID
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Gluc Mutations - M43L, M110L

   Promoter

   T7

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSico Dnmt1

   Plasmid

   #12165

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Insert

   Dnmt1 shRNA (Dnmt1 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is expressed from this plasmid as a marker, …

   Promoter

   Available since

   June 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

   Plasmid

   #166798

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   LL100EE

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCP2

   Plasmid

   #155499

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   DCP2 (DCP2 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE2 GFP-Rtn4HD

    Plasmid

    #86686

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mKate1.3-Fibrillarin-7

    Plasmid

    #56084

    Purpose

    Localization: Nucleoli, Excitation: 588, Emission: 635

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    mKate1.3

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. qTAG-N-Blast-sTagRFP-TUBA1B

    Plasmid

    #207768

    Purpose

    Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET28-p53 (1-90) (72P)

    Plasmid

    #62080

    Purpose

    Expression of human p53 (1-90) (72P) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD/PP residues 1-90; 72P

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pLEX304-ARF5-mNeonGreen

    Plasmid

    #162025

    Purpose

    Expression of tagged ARF WT

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    ARF5 (ARF5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-1574/+120

    Plasmid

    #35539

    Depositor

    Greg Goodall

    Article

    Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

    Insert

    promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    none

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW TetO HA-AMPKa1 AS

    Plasmid

    #69825

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

    Plasmid

    #58275

    Purpose

    mammalian expression of human RdgBbeta splice variant 1

    Depositor

    Shamshad Cockcroft

    Article

    Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

    Insert

    RdgBbeta (PITPNC1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pXPR_BRD111

    Plasmid

    #78166

    Purpose

    Expresses Cas9 with the pLX311 backbone

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pFUW-tetO-GFI1

    Plasmid

    #139813

    Purpose

    doxycycline-inducible expression of Human GFI1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    GFI1 (GFI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P2L- Please see depositor comment below

    Promoter

    TRE-mCMV

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFUW-tetO-BCL6

    Plasmid

    #139816

    Purpose

    doxycycline-inducible expression of Human BCL6 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T190A, E241G, L314M- please see depositor comments

    Promoter

    TRE-mCMV

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Clover-Mito-7

    Plasmid

    #56529

    Purpose

    Localization: Mitochondria, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pLV V5-TbID-emerin

    Plasmid

    #175099

    Purpose

    Lentiviral expression of V5-TbID-tagged mouse Emd

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5-TbID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

    Plasmid

    #135565

    Purpose

    An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    miR-30 rat SST
    Nuc-EYFP
    

    Use

    AAV

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1 and hSYN1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. SMN1-1

    Plasmid

    #156022

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SMN1 (SMN1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNeuLite PEA3 mt

    Plasmid

    #16248

    Depositor

    Mien-Chie Hung

    Article

    Xing et al Nat Med. 2000 Feb . 6(2):189-95.

    Insert

    Her-2/neu promoter (ERBB2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    PEA3 mt: PEA3 motif AGGAAG changed to AGCTCG

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pSAM2_mCherry_Mesp2

    Plasmid

    #72693

    Purpose

    Doxycyline regulated expression of Mesp2 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Mesp 2 (Mesp2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-Doxycycline

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PARD6A_PDZ_1

    Plasmid

    #103946

    Purpose

    Protein expression and purification of TAX INTERACTION PROTEIN PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PARD6A (PARD6A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7-7 asyn S87E

    Plasmid

    #36059

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

    Insert

    alpha-synuclein S87E (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S87E

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-Gluc-EL222-his

    Plasmid

    #172097

    Purpose

    Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

    Depositor

    Avi Schroeder

    Article

    Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

    Insert

    Gaussia Luciferase - EL222
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gluc mutations - M43L, M110L

    Promoter

    T7

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge