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We narrowed to 24,275 results for: CHI

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Showing: 5161 - 5180 of 24275 results

  3. pRSF-MjCouRS

    Plasmid
    #229066
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024
    Insert
    Methanococcus jannaschii tRNA synthetase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    229066-plasmid-map-sequence-id-459819

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  5. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available Since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47948-plasmid-map-sequence-id-68812

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  16. pEVOL-ABK

    Plasmid
    #126035
    Purpose
    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:
    Inserts
    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    araBAD promoter, glnS promoter, and proK promoter
    Available Since
    Sept. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    126035-plasmid-map-sequence-id-244699

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  18. pDule-Tet2.0

    Plasmid
    #85496
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85496-plasmid-map-sequence-id-169865

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  20. pBpaRS3tRNA

    Plasmid
    #197102
    Purpose
    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d
    Insert
    TyrRS variant, amber suppressor tRNA
    Expression
    Bacterial
    Mutation
    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197102-plasmid-map-sequence-id-388022

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Showing: 5161 - 5180 of 24275 results