We narrowed to 11,757 results for: pcDNA

1. pcDNA5/FRT/TO FLAG-RPS28

   Plasmid

   #203156

   Purpose

   N-FLAG-RPS28 in inducible pcDNA5 vector for Flp-In integration

   Depositor

   Susan Baserga

   Article

   Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):198

   Insert

   RPS28 (RPS28 Human)
   

   Use

   Tags

   1X FLAG tag with GGGGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-GST-EGFP-GPIDAF

   Plasmid

   #213722

   Purpose

   For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF driven by an SV40 promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   Tags

   V5-tag-GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-GST-EGFP-GPICEAM7

   Plasmid

   #213723

   Purpose

   For overexpression of a gene, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 driven by an SV40 promoter.

   Depositor

   Qilai Huang

   Article

   Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

   Insert

   EGFP
   

   Use

   Tags

   V5-tag-GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

   Plasmid

   #214614

   Purpose

   adapter for RTK split TEV recruitment assays

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   3xSH2 domains of GRB2-CTEV (GRB2 Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_ERBB4-NTEV-tcs-GV-2xHA

   Plasmid

   #214613

   Purpose

   ERBB4 receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   ERBB4-NTEV (ERBB4 Human)
   

   Use

   Tags

   TEV-Gal4-VP16-2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_ERBB3-NTEV-tcs-GV-2xHA

   Plasmid

   #214611

   Purpose

   ERBB3 receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   ERBB3-NTEV (ERBB3 Human)
   

   Use

   Tags

   TEV-Gal4-VP16-2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_EGFR-NTEV-tcs-GV-2xHA

   Plasmid

   #214610

   Purpose

   EGFR receptor for split TEV assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   EGFR-NTEV (EGFR Human)
   

   Use

   Tags

   TEV-Gal4-VP16-2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-TST-EGFP-GPIDAF

   Plasmid

   #213712

   Purpose

   For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   Tags

   Twin-strep-tag and V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-TST-EGFP-GPIBY55

   Plasmid

   #213711

   Purpose

   For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   Tags

   Twin-strep-tag and V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-VcnTS-E1015A-E1021A

    Plasmid

    #213417

    Purpose

    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A-E1021A (VCL Chicken, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-VcnCS-E1015A-E1021A

    Plasmid

    #213414

    Purpose

    Vinculin conformation sensor (VcnCS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1015A-E1021A (VCL Chicken, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 and 1021 to a…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-FRT-TO-NUbo-E3(63)-FLAG

    Plasmid

    #212808

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(63)
    

    Use

    Tags

    FLAG and NUbo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-FRT-TO-NUbo-FLAG

    Plasmid

    #212797

    Purpose

    Constitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-C17orf53_1-241-mCer

    Plasmid

    #211443

    Purpose

    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Deletion of aa 242-646

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-C17orf53_1-241-mVen

    Plasmid

    #211444

    Purpose

    mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Deletion of aa 242-646

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT-TO-NUbo-E3(48)-FLAG

    Plasmid

    #212802

    Purpose

    Constitutive/Doxycycline-inducible expression of NUbo-E3(48)-FLAG in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48)
    

    Use

    Tags

    FLAG and NUbo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT-TO-Myc-E3(1)-CUbo

    Plasmid

    #212799

    Purpose

    Constitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Tags

    CUbo and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-CRISPR-Puro-sgRPL3-62

    Plasmid

    #208647

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-CRISPR-Puro-sgRPL3-56

    Plasmid

    #208979

    Purpose

    pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

    Depositor

    Quanfu Ma

    Insert

    sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6 in tandem

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRAPa(R150A)-3xFLAG-pCDNA

    Plasmid

    #201966

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    R150A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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