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pcDNA5-FRT-TO-NOD2-EGFP

Plasmid

#131207

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Publication

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD2 (NOD2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6 (1-373)m1

Plasmid

#27174

Purpose

For mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.

Depositor

Ron Prywes

Publication

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains only aa 1-373 Amino acids 315–317 are c…

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
Flag pLJM1 Rap2A

Plasmid

#19311

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RAP2A, member of RAS oncogene family (RAP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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syntaxin3-myc-myc-His

Plasmid

#12372

Depositor

Thomas Weimbs

Publication

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector

Available since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBABE-puro SHP2 WT

Plasmid

#8329

Depositor

Ben Neel

Publication

Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

Insert

SHP2 (PTPN11 Human)

Use

Retroviral

Expression

Mammalian

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hsyn-ASAP5-WPRE

Plasmid

#225709

Purpose

Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

Depositor

Michael Lin

Publication

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Insert

ASAP5

Use

AAV

Promoter

human synapsin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EGFP-RILP

Plasmid

#110498

Purpose

Fluorescent RILP

Depositor

Terence Dermody, Bernardo Mainou

Publication

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

RILP (RILP Human)

Tags

EGFP

Expression

Mammalian

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Publication

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Expression

Mammalian

Promoter

U6 and mouse U6

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCAGGS-5HA-mISG15

Plasmid

#12444

Depositor

Dong-Er Zhang

Publication

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15

Tags

5xHA

Expression

Mammalian

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBJ5 hBRG1

Plasmid

#17873

Depositor

Jerry Crabtree

Publication

Khavari et al Nature. 1993 Nov 11. 366(6451):170-4.

Insert

Brg (SMARCA4 Human)

Expression

Mammalian

Mutation

R1608Q

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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VP16-ER beta (long)

Plasmid

#11352

Depositor

Donald McDonnell

Publication

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCS2-Gal4ff.zf1

Plasmid

#61392

Purpose

mRNA synthesis of Gal4ff codon modified for zebrafish

Depositor

Harold Burgess

Publication

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Gal4ff.zf1

Use

Zebrafish expression

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEX-2T-IQGAP1

Plasmid

#30107

Depositor

David Sacks

Publication

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

GST

Expression

Bacterial

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBabe-TetCMV-puro-mCherry-PA-Rac1

Plasmid

#22035

Purpose

Expression of photoactivatable Rac1

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCherry-PA-Rac1 (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Promoter

Tet-CMV

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ADORA3-Tango

Plasmid

#66212

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADORA3 (ADORA3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDS257

Plasmid

#34971

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBMN HAYFP-LOV24

Plasmid

#49570

Purpose

regulate protein stability with light

Depositor

Thomas Wandless

Publication

Bonger et al ACS Chem Biol. 2014 Jan 17;9(1):111-5. d

Insert

YFP-LOV24

Use

Retroviral

Tags

HA and YFP

Mutation

delete the last 3 aa from AsLOV2, with I532A muta…

Promoter

LTR

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGEX6P1-3xMBT

Plasmid

#46987

Purpose

Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

Depositor

Or Gozani

Publication

Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

Insert

Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)

Tags

GST and PreScission cleavage site

Expression

Bacterial

Mutation

contains amino acids 190–530 of accession NP_0562…

Promoter

tac

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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