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Thy1-Brainbow-2.1 R

Plasmid

#18727

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pCH1/RAD51o

Plasmid

#102562

Purpose

Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

Depositor

Maria Spies

Article

Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

Inserts

Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
E. coli groE operon

Expression

Bacterial

Mutation

Codon optimized for E.coli Expression

Promoter

T7

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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C1-F-tractin-mCherry

Plasmid

#155218

Purpose

Monitor global F-actin distribution

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEntr-GATA4flbio

Plasmid

#32937

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

GATA4 (Gata4 Mouse)

Use

Gateway Cloning

Tags

flag-bio

Promoter

none

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV LIC cloning vector (1R)

Plasmid

#29664

Depositor

Scott Gradia

Type

Empty backbone

Tags

Strep II and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pAL101

Plasmid

#22474

Purpose

Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 SENP2

Plasmid

#13382

Depositor

Mary Dasso

Article

Hang et al J Biol Chem. 2002 May 31. 277(22):19961-

Insert

SENP2 (SENP2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-cyan pre-eGRASP(p32)

Plasmid

#111589

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Venus-Flag (865)

Plasmid

#40998

Depositor

Jonathon Pines

Article

Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

Type

Empty backbone

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-NL1B

Plasmid

#15261

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert B (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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integrin beta6 EGFP-N3

Plasmid

#13593

Depositor

Dean Sheppard

Insert

integrin beta 6 (ITGB6 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Paf1

Plasmid

#11059

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Paf1 (PAF1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pYPQ144

Plasmid

#69296

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Promoter

UbC

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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Cterminal HA WDR59 pRK5

Plasmid

#46328

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR59 (WDR59 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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HA-Mob

Plasmid

#32835

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mob (MOB1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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