We narrowed to 15,742 results for: grna

1. p426_Cas9_gRNA-ARS1014a

   Plasmid

   #87396

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKDsgRNA-trmI

   Plasmid

   #89955

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   trmI gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKDsgRNA-pfkB

   Plasmid

   #102284

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   pfkB gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p426_Cas9_gRNA-YOLCd1b

   Plasmid

   #87401

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YOLCd1b sequence GACTAGTTAAGCGAGCATGT in yeast chromosome 15.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting YOLCd1b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKDsgRNA-xylA

   Plasmid

   #89964

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   xylA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKDsgRNA-lpoB

   Plasmid

   #89959

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   lpoB gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330-sgRNA_Dgcr8_3

   Plasmid

   #73527

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pX330-sgRNA_Dgcr8_4

   Plasmid

   #73528

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pX330-sgRNA_Dgcr8_2

   Plasmid

   #73526

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pHU6_gRNA_Ch10-for1

    Plasmid

    #81210

    Purpose

    for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHU6_gRNA_Ch10-rev1

    Plasmid

    #81208

    Purpose

    for targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7-gRNA_zebrafish_mmp21

    Plasmid

    #72890

    Purpose

    plasmid to generate guideRNA against mmp21 gene in zebrafish

    Depositor

    Nicholas Katsanis

    Article

    Perles et al J Med Genet. 2015 Dec;52(12):840-7. doi:

    Insert

    zebrafish mmp21 (mmp21 Zebrafish)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7 RNA pol

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_rtTA_BsmB-HNRNPK_sgRNA_4

    Plasmid

    #242899

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB_rtTA_BsmB-HNRNPK_sgRNA_1

    Plasmid

    #242897

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB_rtTA_BsmB-HNRNPK_sgRNA_3

    Plasmid

    #242898

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSEVA63_sgRNA-B0_M13ps

    Plasmid

    #231421

    Purpose

    M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-B0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEVA63_sgRNA-B1_M13ps

    Plasmid

    #231422

    Purpose

    M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-B1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HaloXRCC4 sgRNA

    Plasmid

    #207606

    Purpose

    sgRNA for the insert of the HaloTag at the endogenous loci of XRCC4.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    TACTGGGTTCAGAAACAAGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX330-sgRNA006_CTCF

    Plasmid

    #232928

    Purpose

    Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9 (Ctcf S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX459-sgRNA047_Podxl

    Plasmid

    #232932

    Purpose

    Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Podxl S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EHMT1_exon 3_gRNA

    Plasmid

    #228809

    Purpose

    CRISPR targeting of human EHMT1

    Depositor

    Shravanti Rampalli

    Article

    Biligiri et al PLOS Biology 22(11): e3002871.

    Insert

    gRNA targeting EHMT1 exon 3 (EHMT1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. esgRNA_NbATML1-1pro

    Plasmid

    #231151

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1pro

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbATML1-1pro
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. esgRNA_NbSTM-5'UTR

    Plasmid

    #231150

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTR

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbSTM-5?UTR
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSEVA19_sgRNA-C1_M13ps

    Plasmid

    #231411

    Purpose

    M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-C1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSEVA26_sgRNA-2_M13ps

    Plasmid

    #231412

    Purpose

    M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSEVA26_sgRNA-A0_M13ps

    Plasmid

    #231413

    Purpose

    M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Insert

    sgRNA-A0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSEVA19_LacI_pLac-sgRNA-1

    Plasmid

    #231408

    Purpose

    sgRNA-1 expression from an IPTG-inducible promoter.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    sgRNA-1
    LacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSEVA19_AraC_pBAD-sgRNA-1

    Plasmid

    #231407

    Purpose

    sgRNA-1 expression from an arabinose-inducible promoter.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    sgRNA-1
    AraC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSEVA26_sgRNA-A1_M13ps

    Plasmid

    #231414

    Purpose

    M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-A1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSEVA19_sgRNA-C0_M13ps

    Plasmid

    #231410

    Purpose

    M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-C0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge