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We narrowed to 1,621 results for: nif

Showing: 751 - 780 of 1621 results

  1. pOpen-T4 PGD

    Plasmid
    #165550
    Purpose
    T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T4 PGD (T4 Endonuclease V)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165550-plasmid-map-sequence-id-339867

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  2. pOpen-SP6rpol

    Plasmid
    #165516
    Purpose
    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    SP6 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165516-plasmid-map-sequence-id-338647

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  3. pOpen-PstIM

    Plasmid
    #165536
    Purpose
    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    M.PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165536-plasmid-map-sequence-id-339126

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  4. pOpen-TEV protease

    Plasmid
    #165509
    Purpose
    Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    TEV protease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165509-plasmid-map-sequence-id-338590

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  5. pOpen-DpnIR

    Plasmid
    #165529
    Purpose
    Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    DpnI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165529-plasmid-map-sequence-id-339077

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  6. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

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  7. pOpen-ALPI

    Plasmid
    #165555
    Purpose
    CIAP reporter; can be used in spectrophotometric or luminescence assay when appropriate substrate is used
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    CIP (calf intestinal phosphatase)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165555-plasmid-map-sequence-id-339907

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  8. pOpen-T7EI

    Plasmid
    #165540
    Purpose
    DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T7EI (T7 Endonuclease I)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165540-plasmid-map-sequence-id-339262

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  9. pOpen-9N7polA (CT)

    Plasmid
    #165502
    Purpose
    9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    9 degrees N-7 DNA Polymerase chain terminating
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165502-plasmid-map-sequence-id-338438

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  10. pOpen-EcoRIM

    Plasmid
    #165515
    Purpose
    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    M.EcoRI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165515-plasmid-map-sequence-id-338645

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  11. pOpen-HSlig4

    Plasmid
    #165503
    Purpose
    Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    DNA Ligase IV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165503-plasmid-map-sequence-id-338457

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  12. L1B39 - pMUTE-Bxb1-tUBQ10

    Plasmid
    #219704
    Purpose
    Level 1 pMUTE-Bxb1-tUBQ10, for stomata specific expression of Bxb1 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pMUTE:Bxb1:tUBQ10
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219704-plasmid-map-sequence-id-435734

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  13. pOpen-GBDpol

    Plasmid
    #165501
    Purpose
    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Pyrococcus Sp. Heat-Stable DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165501-plasmid-map-sequence-id-338429

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  14. pOpen-K12polLF

    Plasmid
    #165511
    Purpose
    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    DNA Polymerase I, Large (Klenow) Fragment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165511-plasmid-map-sequence-id-338596

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  15. pOpen-T5gene122

    Plasmid
    #165544
    Purpose
    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T5 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165544-plasmid-map-sequence-id-339280

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  16. pOpen-RNAse H

    Plasmid
    #165575
    Purpose
    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    RNAse H
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165575-plasmid-map-sequence-id-340983

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  17. pOpen-SfiIR (cDNA)

    Plasmid
    #165528
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    SfiI (used to create cDNA libraries)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165528-plasmid-map-sequence-id-339067

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  18. pOpen-Pfulig

    Plasmid
    #165525
    Purpose
    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Pfu DNA Ligase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165525-plasmid-map-sequence-id-338923

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  19. L0-T12 GG2-ER-tagBFP-tUBQ10-GG3

    Plasmid
    #219695
    Purpose
    Level 0 to construct history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    GG2-ER-tagBFP-tUBQ10-GG3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219695-plasmid-map-sequence-id-435727

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  20. L1P139 - pSPCH-PhiC31-tUBQ1

    Plasmid
    #219703
    Purpose
    Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH:PhiC31:tUBQ1
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219703-plasmid-map-sequence-id-435733

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  21. L0-T15 BpiI-p35s-BpiI

    Plasmid
    #219698
    Purpose
    Level 0 to insert p35S promoter to history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    BpiI-p35s-BpiI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219698-plasmid-map-sequence-id-435729

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  22. TGN38 EGFP

    Plasmid
    #128148
    Purpose
    Expression of TGN38 amino acids 304-357
    Depositor
    Jennifer Lippincott-Schwartz
    Insert
    TGN38 (Tgoln2 Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Contains amino acids 304-357
    Promoter
    Available since
    Nov. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128148-plasmid-map-sequence-id-251067

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  23. pOpen-THEAQpolA

    Plasmid
    #165512
    Purpose
    DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Taq DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165512-plasmid-map-sequence-id-339400

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  24. pOpen-T7gene5

    Plasmid
    #165534
    Purpose
    DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T7 DNA Polymerase (unmodified)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165534-plasmid-map-sequence-id-339124

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  25. pOpen-T3gene1

    Plasmid
    #165576
    Purpose
    DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T3 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165576-plasmid-map-sequence-id-340988

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  26. pOpen-dbh

    Plasmid
    #165513
    Purpose
    Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Sulfolobus DNA Polymerase IV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165513-plasmid-map-sequence-id-338598

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  27. L1P137 - pAHP6-PhiC31-NLS-tUBQ1

    Plasmid
    #219702
    Purpose
    Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6:PhiC31-NLS:tUBQ1
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219702-plasmid-map-sequence-id-435732

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  28. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  29. pOpen-lambda red operon

    Plasmid
    #165521
    Purpose
    Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    lambda red operon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165521-plasmid-map-sequence-id-338838

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  30. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165522-plasmid-map-sequence-id-350938

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Showing: 751 - 780 of 1621 results