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Showing: 1251 - 1300 of 9468 results

  1. pJK282

    Plasmid
    #71138
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes histidine-59 to aspartate; additional N-t…
    Promoter
    T7
    Available Since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71138-plasmid-map-sequence-id-131037

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  2. pJK281

    Plasmid
    #71137
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes histidine-59 to phenylalanine; additional…
    Promoter
    T7
    Available Since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71137-plasmid-map-sequence-id-131036

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  3. pJK280

    Plasmid
    #71136
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes histidine-89 to phenylalanine; additional…
    Promoter
    T7
    Available Since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71136-plasmid-map-sequence-id-131035

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  4. pJK175

    Plasmid
    #71132
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7
    Available Since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71132-plasmid-map-sequence-id-131032

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  12. IgK-IFN-luc

    Plasmid
    #14886
    Purpose
    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression
    Depositor
    David Baltimore
    Article
    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.
    Insert
    IgK-IFN (IFNB1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Available Since
    May 1, 2007
    Availability
    Academic Institutions and Nonprofits only
    14886-plasmid-map-sequence-id-238489

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  14. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  15. pKEK3235

    Plasmid
    #234107
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234107-plasmid-map-sequence-id-467990

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  17. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

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  26. pEN41A-T38S38

    Plasmid
    #49524
    Purpose
    Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    constitutive TetR38
    Promoter
    Ptb38
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49524-plasmid-map-sequence-id-73916

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  32. pJEC626

    Plasmid
    #174367
    Purpose
    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.
    Depositor
    James Chappell
    Article
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23106
    Available Since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174367-plasmid-map-sequence-id-345703

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  36. pYSD353

    Plasmid
    #253045
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, FLAG-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Jurić et al bioRxiv 2025.08.19.671047
    Insert
    Anti-GFP nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253045-plasmid-map-sequence-id-507359

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  37. pYSD354

    Plasmid
    #253046
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, MYC-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Jurić et al bioRxiv 2025.08.19.671047
    Insert
    Anti-GFP nanobody 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253046-plasmid-map-sequence-id-507360

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  38. pYSD322

    Plasmid
    #253037
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-HA Frankenbody, MYC-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Jurić et al bioRxiv 2025.08.19.671047
    Insert
    igG 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253037-plasmid-map-sequence-id-507342

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  39. pYSD325

    Plasmid
    #253038
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Protein A ZZ domain, HA-tagged 649-stalk yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Jurić et al bioRxiv 2025.08.19.671047
    Insert
    Protein A 649-stalk anchor
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253038-plasmid-map-sequence-id-507341

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  40. pYSD316

    Plasmid
    #253035
    Purpose
    Encodes the MFalpha1 PrePro-app8 signal peptide, Anti-GFP nanobody, HA-tagged Aga1 deltaN181AA yeast surface display anchor protein in a yeast expression vector with pTEF1, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Jurić et al bioRxiv 2025.08.19.671047
    Insert
    Anti-GFP nanobody -Aga1
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253035-plasmid-map-sequence-id-507338

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  41. CPAlpha1Beta2(R15A,Y79A) pBJ2304

    Plasmid
    #253114
    Purpose
    pBJ2304 Cooper Lab. Bacterial expression of His-tagged mouse CP heterodimer (alpha1, beta2 isoforms). Two point mutations of the beta2 subunit (R15A, Y79A). Little to no binding of CPI motifs.
    Depositor
    John Cooper
    Article
    Edwards et al Nat Commun. 2015 Sep 28;6:8415. doi: 10.
    Insert
    Mouse capping protein alpha1 beta2 (R15A,Y79A). His-tagged.
    Tags
    His
    Expression
    Bacterial
    Mutation
    CP beta2 (R15 to A, Y79 to A).
    Promoter
    T7 lac
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    253114-plasmid-map-sequence-id-504388

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  42. pMKR07

    Plasmid
    #240097
    Purpose
    A CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native host
    Depositor
    Adrian Keatinge-Clay
    Article
    Wang et al Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ermE* promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240097-plasmid-map-sequence-id-478442

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  43. pKEK3220

    Plasmid
    #234099
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234099-plasmid-map-sequence-id-468002

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  44. pKEK3237

    Plasmid
    #234091
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234091-plasmid-map-sequence-id-467987

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  45. pKEK3400

    Plasmid
    #234084
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfGFP
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    S30R/Y39N/N105T/Y145F/I171V/A206V
    Promoter
    pJ23100-BsaI
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234084-plasmid-map-sequence-id-464936

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  48. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

    Plasmid
    #201534
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB3
    Use
    Synthetic Biology
    Mutation
    rhaS W190G
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201534-plasmid-map-sequence-id-406801

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  50. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

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Showing: 1251 - 1300 of 9468 results