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Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS2115
Plasmid#49140PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-emGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57.1 _Gata6
Plasmid#73537PurposeInducible lentiviral expression of Gata6DepositorInsertGata6 (Gata6 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Synaptojanin 1-145
Plasmid#22291DepositorInsertSynaptojanin 1_145 (SYNJ1 Human)
UseTagsFlagExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pEMS1985
Plasmid#49116PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-iCre
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1986
Plasmid#49117PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-iCre-WPRE
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pEGFP-LC3-K51A
Plasmid#155265PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationLysine 51 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3-R70A
Plasmid#155264PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagspEGFPExpressionMammalianMutationArginine 70 to AlaninePromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2cl-FLAG
Plasmid#97077PurposeExpresses CARMA2cl/CARD14 isoform 2 in mammalian cellsDepositorInsertCARD14 isoform 2 (CARD14 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMS2116
Plasmid#49141PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-emGFP
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
SL536
Plasmid#49942PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertRHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2cl-HA
Plasmid#97195PurposeExpresses CARMA2cl/CARD14 isoform 2 in mammalian cellsDepositorInsertCARD14 isoform 2 (CARD14 Human)
UseTagsHAExpressionMammalianMutationnonePromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
SL539
Plasmid#49948PurposeExpresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding siteDepositorInsertRHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
UseTALENTagsExpressionMutationPromoterEF1aAvailable sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)
Plasmid#226578PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationF196A, L199A, F200A, L203A, Y206A, L210A, L214A, …PromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRE3G_hRTN3E-Halo
Plasmid#216252PurposeTet-inducible hRTN3E (long form) expression vector, expresses hRTN3E-TEV-Halo, Lentiviral vectorDepositorInsertreticulon-3 isoform e (RTN3 Human)
UseLentiviralTagsHaloTagExpressionMutationPromoterTRE3GAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only