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Plasmid#160112PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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pBEAST_J23101-vdh
Plasmid#128134PurposeThe cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK287
Plasmid#71120PurposeProduces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCP-Luc (MCP-1 promoter in pGL3-basic)
Plasmid#40324DepositorInsertMCP-1 promoter (Ccl2 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationcontains MCP-1 promoter region through the nucleo…PromoterMCP-1Available sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG_PCP-T5Exo
Plasmid#214736Purposeencodes T5 exonuclease with a PP7 coat protein fused to the N terminusDepositorInsertCAG_PCP-T5Exo_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2CP-VPg(FCV)
Plasmid#167314PurposeTemplate pDNA to prepare CaVT mRNADepositorInsertCaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES_mScarleti_Sec23A
Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsUseTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG MCP-VP64
Plasmid#92357PurposeCAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.DepositorInsertMCP-VP64
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7 oligo-library based
Plasmid#158199PurposeCassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of PP7 from the oligo library
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbMS2
Plasmid#171701PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK389.AAV
Plasmid#177327PurposeaTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expressionDepositorInsertsSoxS
dCas9
UseCRISPRTagsMCP (MS2 Coat Protein)ExpressionMutationR93A,S101APromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 oligo-library based
Plasmid#158200PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.DepositorInsert10 binding sites of MS2 from oligo library experiment
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_YPet-PCP (y-plasmid)
Plasmid#127637PurposeEncodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.DepositorInsertYPet
UseSynthetic BiologyTagsRNA binding peptide: PP7 coat protein (PCP)ExpressionBacterialMutationPromoterpJ21306Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCMV-MS2CP-eDHFR
Plasmid#167309PurposeTemplate pDNA to prepare a split CaVT component mRNADepositorInsertMS2CP-eDHFR
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA009
Plasmid#22842DepositorInsertA DNA containing CHLI fragment
UseCotton vigs vectorTagsExpressionBacterialMutationCotton Leaf Crumple Virus Coat Protein (AR1 gene)…PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
GvpA-CaM
Plasmid#223555PurposeFull length gas vesicle coat protein A derived from H. salinarum (GvpA) fused to calmodulin.DepositorInsertpET300-GvpACaM
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VP16
Plasmid#183935PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VP16 (UL48 Synthetic)
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPDK3876(TRV-RNA2-pPEBV-MCS)
Plasmid#149275PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAsDepositorInsertModified TRV RNA2 with PEBV promoter
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
Plasmid#164614PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.DepositorInsertstdMCP-stdHalo-RH1
UseLentiviralTagsHA tag and NLSExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only