We narrowed to 4,313 results for: Pet2

1. pET28-mTDGa.1

   Plasmid

   #81051

   Purpose

   bacterial expression of catalytically dead murine TDG

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TDG (Tdg Mouse)
   

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   asparagine 151 changed to alanine

   Promoter

   T7

   Available Since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-TmCA-P(11)4

   Plasmid

   #185783

   Purpose

   Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   TmCA-P(11)4
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28b-pvdA WT

   Plasmid

   #87098

   Purpose

   expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

   Depositor

   Audrey Lamb

   Article

   Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

   Insert

   pvdA WT
   

   Tags

   his-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-hdNadV

   Plasmid

   #83362

   Purpose

   Expresses nadV from Haemophilus ducreyi in Escherichia coli

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

   Insert

   nadV
   

   Tags

   none

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-NpR5600

   Plasmid

   #45741

   Depositor

   Christopher T Walsh, Emily Balskus

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   NpR5600 (Npun_R5600 Nostoc punctiforme)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pET28a-PAN

   Plasmid

   #200971

   Purpose

   Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

   Depositor

   Yifan Cheng

   Article

   Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

   Insert

   PAN
   

   Tags

   6xHis and 6xHis-thrombin site

   Expression

   Bacterial

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a MDH_PFALCI

   Plasmid

   #204235

   Purpose

   Bacterial expression of unicellular protozoan parasite P. falciparum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDH_PFALCI (PF3D7_0618500 Plasmodium falciparum)
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-nGFPMNase

   Plasmid

   #187826

   Purpose

   express nGFPMNase protein in E coli

   Depositor

   Yikang Rong

   Article

   Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

   Insert

   nGFPMNASE
   

   Tags

   his tag

   Expression

   Bacterial

   Promoter

   T7 Promotor

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a Gx-NL3

   Plasmid

   #169108

   Purpose

   Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc - NanoLuc
   

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28b-PU.1-ETS

    Plasmid

    #85734

    Purpose

    Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272

    Depositor

    Gregory Poon

    Article

    Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

    Insert

    ETS domain of murine PU.1 (residues 167-272) (Spi1 Human, Mouse)
    

    Tags

    6xHis following thrombin cleavage site LVPR|G

    Expression

    Bacterial

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-HA-CPDSalI

    Plasmid

    #38254

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-HsSENP6

    Plasmid

    #71469

    Purpose

    Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 6 (SENP6 Human)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET28-mHP1alpha

    Plasmid

    #181911

    Purpose

    Bacterial protein expression vector for HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET29b-NpR5599

    Plasmid

    #45740

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    NpR5599 (Npun_R5599 Nostoc punctiforme)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pET21a Hbb

    Plasmid

    #26639

    Depositor

    Phoebe Rice

    Article

    Mouw et al Mol Microbiol. 2007 Mar . 63(5):1319-30.

    Insert

    Hbb (hbb Borrelia burgdorferi)
    

    Expression

    Bacterial

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-UVRAG CC

    Plasmid

    #24400

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    UVRAG (UVRAG Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains on the CC domain, amino acids 200-270

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pET29a-hNABP1-C

    Plasmid

    #128311

    Purpose

    Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a - Mtb-TF

    Plasmid

    #111513

    Purpose

    Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    Trigger Factor
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b-HA-CPDSalI

    Plasmid

    #38253

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Tags

    6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21-tVYN-Nt∆

    Plasmid

    #154431

    Purpose

    Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

    Depositor

    Ming Hammond

    Article

    Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

    Insert

    VenusΔC12-NtYcgRΔ-NLucΔ4
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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