We narrowed to 1,322 results for: pET28a

1. pET28a-hfYFP-TEV-mScarlet-i

   Plasmid

   #191196

   Purpose

   Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & mScarlet-I fusion
   

   Use

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-hfYFP-TEV-SAV

   Plasmid

   #191197

   Purpose

   Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-LSSmGFP-TEV-mScarlet-i

   Plasmid

   #191199

   Purpose

   Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & mScarlet-I fusion
   

   Use

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-LSSmGFP-TEV-SAV

   Plasmid

   #191200

   Purpose

   Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

   Plasmid

   #187708

   Purpose

   Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 381-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-380

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)-NEDD4L_W3-W4-HECT (492)

   Plasmid

   #187709

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-491

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-FKBP-IRSp53(pLxIS)

   Plasmid

   #221336

   Purpose

   Overexpression of IRSp53 pLxIS domain in bacteria

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   IRSp53 pLxIS domain (Baiap2 Mouse)
   

   Use

   Tags

   6xHis-4xFKBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-FKBP-MAVS(pLxIS)

   Plasmid

   #221335

   Purpose

   Overexpression of MAVS pLxIS domain in bacteria

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   MAVS pLxIS domain (Mavs Mouse)
   

   Use

   Tags

   6xHis-4xFKBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-FKBP-TRIF(Nterm)

   Plasmid

   #221334

   Purpose

   Overexpression of TRIF Nterm domain in bacteria

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   TRIF Nterm domain (Ticam1 Mouse)
   

   Use

   Tags

   6xHis-4xFKBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-FKBP-GMIP(pLxIS)

    Plasmid

    #221337

    Purpose

    Overexpression of GMIP pLxIS domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    GMIP pLxIS domain (Gmip Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-SnoopTag-SpyTag-(AffiHER2)x3

    Plasmid

    #216281

    Purpose

    Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-SpyTag-(AffiHER2)x3
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a LZ LED 4CA-FLAG

    Plasmid

    #199681

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED 4CA (GPR155 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    C595.604.629.638 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a LZ LED Y551A-FLAG

    Plasmid

    #199680

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED Y551A (GPR155 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Y551 of LYCHOS mutated to A

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a Cdk2ap1ΔN (MT2B2)-MutTER

    Plasmid

    #178035

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    First N-Terminal 27aa are deleted and Changed T(8…

    Promoter

    T7LacI

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PET28a-cpLumiLuc-amylase sensor

    Plasmid

    #186293

    Purpose

    Expression alpha-amylase sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-amylase sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PET28a-cpLumiLuc-HSA sensor

    Plasmid

    #186294

    Purpose

    Expression Human serum albumin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-HSA sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-anticalin (E60amb)-His

    Plasmid

    #168043

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 60 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-anticalin (E143amb)-His

    Plasmid

    #168046

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Glu 143 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-anticalin (S87amb)-His

    Plasmid

    #168044

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Ser 87 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-anticalin (N116amb)-His

    Plasmid

    #168045

    Purpose

    Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Anticalin
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Asn 116 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge