Addgene

We narrowed to 766 results for: xenopus

Showing: 501 - 520 of 766 results

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CMV::TVA/tRFP

Plasmid

#164486

Purpose

Dual CMV promoter plasmid to express TVA and TurboRFP

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Inserts

TVA (CD320 Chicken)
TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

sCMV and sCMV IE94

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

Plasmid

#74101

Purpose

vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Use

Tags

Expression

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBSHSP:Cre;CMV:tdTomato-SceI

Plasmid

#30525

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

Cre recombinase tdTomato

Use

Cre/Lox; Heat shock inducible, i-scei megabase ve…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCS_FLPER(T)

Plasmid

#31131

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP-ER ligand binding domain (ESR1 Budding Yeast, Human)

Use

Tags

Expression

Mammalian

Mutation

full length FLP linked to ligand binding domain o…

Promoter

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pBSHSP:FLP;CMV:tdTomato-SceI

Plasmid

#30523

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

FLP recombinase tdTomato

Use

Flp/frt, heat shock inducible, i-scei megabase ve…

Tags

Expression

Mutation

R415H

Promoter

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Mix.2-lux M-p53BE

Plasmid

#20894

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

-290 Mix.2 M-p53BE promoter luciferase

Use

Tags

Expression

Mammalian

Mutation

point mutation of the p53 binding element (p53BE)…

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pCSP1-HA-xEcto

Plasmid

#20900

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (trim33 Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

contains full unsequenced 3' UTR

Promoter

Available since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

trc

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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Zebrafish lim5 (lhx5)

Plasmid

#74141

Purpose

Basic cDNA clone for zebrafish lim5 (lhx5). Cloned in BS KS+.

Depositor

Igor Dawid

Article

Toyama et al Dev Biol. 1995 Aug;170(2):583-93.

Insert

lhx5 (lhx5 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only
p076 pCS xTRPS1 delN delC320 (M)

Plasmid

#11046

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Tags

Expression

Mutation

aa806-952 of xTRPS1. Two Cys residues in the GAT…

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
p063 pCS xTRPS1 delta N (M)

Plasmid

#11036

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 deltaN mut (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Tags

Expression

Mutation

aa806-1153 of xTRPS1. Two Cys residues in the GA…

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
V22 CS2+ RFPce

Plasmid

#17113

Depositor

Randall Moon

Type

Empty backbone

Use

Xenopus/mammalian/avian/zebrafish

Tags

RFP (N- or C-term)

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta wt pcDNA3

Plasmid

#14753

Purpose

Mammalian expression of human GSK3 beta with HA tag

Depositor

Jim Woodgett

Article

He et al Nature. 1995 Apr 13. 374(6523):617-22.

Insert

GSK3 beta (GSK3B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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mOtop3_pGEMHE

Plasmid

#114676

Purpose

This construct was used to express mouse Otop3 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 3 (Otop3 Mouse)

Use

For generating the mrna, which can highly express…

Tags

Expression

Mutation

Promoter

T7

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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Tg(mpeg1:LifeAct-mRuby)

Plasmid

#166977

Purpose

labels F-actin in macrophages

Depositor

Anna Huttenlocher

Article

Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

Inserts

mpeg1 (mpeg1.1 Zebrafish)
LifeAct (actb1 Frog)

Use

Tags

Lifeact-mRuby and mRuby

Expression

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Use

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-4T1 SHP2 WT

Plasmid

#8322

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

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pOO2-GW

Plasmid

#135097

Purpose

Gateway vector for expression of proteins in Xenopus oocytes

Depositor

Wolf Frommer

Article

Chen et al Nature. 2010 Nov 25;468(7323):527-32. do

Type

Empty backbone

Use

Bacterial expression vector for generating sp6 tr…

Tags

Expression

Mutation

Promoter

SP6

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mOtop2_pGEMHE

Plasmid

#114675

Purpose

This construct was used to express mouse Otop2 in Xenopus laevis oocytes

Depositor

Emily Liman

Article

Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

Insert

Otopetrin 2 (Otop2 Mouse)

Use

For generating the mrna, which can highly express…

Tags

Expression

Mutation

Promoter

T7

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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