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pcmv3-STING-C64S,C65S-FLAG

Plasmid

#198186

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

The cysteine in STING at position 64/65 mutates t…

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Rem2 [N324/2]

Plasmid

#190312

Purpose

Mammalian Expression Plasmid of anti-Rem2 (Rat). Derived from hybridoma N324/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Rem2 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Rem2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

Plasmid

#115787

Purpose

Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

FZD4 (FZD4 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS3

Plasmid

#170854

Purpose

AAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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Synaptotagmin-7 scFv [N275/14]

Plasmid

#211608

Purpose

Mammalian Expression Plasmid of Synaptotagmin-7 scFv. Derived from hybridoma N275/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Synaptotagmin-7 (Rattus norvegicus) recombinant mouse scFv. (Syt7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-HA-GFI1B

Plasmid

#125599

Purpose

doxycycline-inducible expression of human GFI1B (HA tagged) in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

Growth Factor Independent 1B transcriptional repressor (GFI1B Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shCrh

Plasmid

#132715

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shCrh (Crh Rat)

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin 1

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Aurora_CA

Plasmid

#98170

Purpose

slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora_C128A gene

Use

Tags

mCherry

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

Promoter

CMV (+enhancer)

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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WRN scFv [N428/12]

Plasmid

#206784

Purpose

Mammalian Expression of WRN scFV. Derived from hybridoma N428/12 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

WRN (Homo sapiens) recombinant scFV (WRN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-GFP

Plasmid

#178713

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-dTom

Plasmid

#178715

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-STAT3

Plasmid

#195575

Purpose

Expresses STAT3

Depositor

Kevin Park

Article

Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer and promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Beta4-spectrin scFv [N393/2]

Plasmid

#190550

Purpose

Mammalian Expression of Beta4-spectrin scFV. Derived from hybridoma N393/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Beta4-spectrin (Homo sapiens) recombinant scFV (SPTBN4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC1 [1F1R]

Plasmid

#177437

Purpose

Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9-Puro HLAG-2A-sgRNA

Plasmid

#182551

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2A-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2

Plasmid

#170853

Purpose

AAV backbone for Cre-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236A-pcDNA3.1

Plasmid

#85764

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236A

Promoter

CMV

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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USPL1cat-C236S-pEYFP_C1

Plasmid

#85767

Purpose

mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236S

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

catalytic domain (aa 212-514), catalytic inactiv…

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236S-pcDNA3.1

Plasmid

#85763

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236S

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Gcn5(D608A)

Plasmid

#63707

Purpose

retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

D608A mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only