We narrowed to 6,725 results for: SIM;

1. pMEL16

   Plasmid

   #107922

   Purpose

   HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-FLEX-ArchT-GFP

   Plasmid

   #58851

   Purpose

   AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-GFP
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330 Rosa26 Gu1 (LH416)

   Plasmid

   #99629

   Purpose

   Plasmid used to target the wildtype Rosa26 allele with Cas9

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-puro-Halo-hMRE11(wt)

   Plasmid

   #208393

   Purpose

   To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   Human MRE11 (MRE11 Human)
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   H129N

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-puro-Halo-hMRE11(H129N)

   Plasmid

   #208394

   Purpose

   To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Insert

   Human MRE11 (MRE11 Human)
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   H129N

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGem-nHyPer:cAPX

   Plasmid

   #84736

   Purpose

   Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   APX1 (APX1 Mustard Weed)
   HyPer2 targeted to nucleus
   

   Use

   Tags

   NLS-SV40

   Expression

   Plant

   Mutation

   Promoter

   pFMV/p35S

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMEL10

   Plasmid

   #107916

   Purpose

   URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETM33_CALM1_EF-hand

   Plasmid

   #178468

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CALM1_EF-hand (CALM1 Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmKO-imp α

   Plasmid

   #200082

   Purpose

   Expresses mKO-tagged human importinα in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

   Insert

   importin α (KPNA2 Human)
   

   Use

   Tags

   mKO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-MED4

    Plasmid

    #15425

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED4 (MED4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. FLAG-MED10

    Plasmid

    #15417

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED10 (Med10 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-45b-HOXD13-IDR-mCherry

    Plasmid

    #145280

    Purpose

    Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCerulean-PCNA-PuroR

    Plasmid

    #215063

    Purpose

    Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PCNA (PCNA Human)
    

    Use

    Retroviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1α-mouse-full-length-CHMP3-HA

    Plasmid

    #154176

    Purpose

    expresses mouse CHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3 (Chmp3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-HOXD13-IDR-mCherry-CRY2

    Plasmid

    #145258

    Purpose

    Mammalian Expression of Opto HOXD13 IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13wt-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSCRET2(#323)

    Plasmid

    #184058

    Purpose

    cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

    Insert

    6myc-CRE-ERT2
    

    Use

    Synthetic Biology

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMEL17

    Plasmid

    #107923

    Purpose

    TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-RUNX2-IDR-mCherry-CRY2-NLS

    Plasmid

    #145267

    Purpose

    Mammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2wt-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYpet-Optop38i

    Plasmid

    #89749

    Purpose

    Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i3
    

    Use

    Fluorescent protein

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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