We narrowed to 32,647 results for: CMV

1. pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

   Plasmid

   #216322

   Purpose

   Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA

   Plasmid

   #74306

   Purpose

   Bicistronic Cre-dependent expression of TRPV4 & ferritin in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Inserts

   TRPV4 (Trpv4 Rat)
   ferritin (FTL and HTL1) (FTL, FTH1 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG and p2A

   Expression

   Mammalian

   Promoter

   CMV (after cre recombination)

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

   Plasmid

   #118702

   Purpose

   lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   wt , SLC7A11 sh926R

   Available Since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120297

   Purpose

   AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

   Plasmid

   #221080

   Purpose

   SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEX-CMV-SP-YFP-STM2(15-746) EF hand D80A

   Plasmid

   #18864

   Depositor

   Tobias Meyer

   Article

   Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

   Insert

   Stromal interaction molecule 2 (STIM2 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   EF hand mutant: D80A. YFP inserted between signal…

   Available Since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense

   Plasmid

   #46835

   Purpose

   Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]

   Depositor

   Phil Sharp

   Article

   Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

   Plasmid

   #197506

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

   Promoter

   CMV and T7

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

   Plasmid

   #197024

   Purpose

   Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized OsCas12f1
   

   Expression

   Mammalian

   Promoter

   CBh, CMV, hU6

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2

    Plasmid

    #210019

    Purpose

    tetO-OKS (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + Sox2)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2 (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO-mini-CMV

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362

    Plasmid

    #31354

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 1047-1362 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid

    #113692

    Purpose

    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    NLS

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid

    #26797

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
    

    Expression

    Mammalian

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. p6347 MSCV-CMV-Flag-HA-Brd4-444-722

    Plasmid

    #31353

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 444-722 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362

    Plasmid

    #31355

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    Brd4 1224-1362 (BRD4 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

    Plasmid

    #208972

    Purpose

    Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169591

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin-2

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

    Plasmid

    #199361

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOstar-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

    Plasmid

    #46836

    Purpose

    Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4

    Plasmid

    #193345

    Purpose

    tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Sox2-17-t2a-Klf4 (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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