We narrowed to 5,602 results for: pCas

1. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2xFKBP-smFP(myc)-KDEL

   Plasmid

   #173021

   Purpose

   FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   2xFKBP-smFP(myc)-KDEL
   

   Use

   Tags

   FKBP and smFP(myc) non-fluorescent

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCPmacroH2A.1-GS3

   Plasmid

   #204759

   Purpose

   Expression of Cas9 and human macroH2A.1

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   macroH2A1 (MACROH2A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CXN-BCL-6 ZFΔ (pCXN2-BCL6-ZFdel)

   Plasmid

   #40347

   Depositor

   Alexander Dent

   Article

   Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

   Insert

   BCL-6 ZFΔ (BCL6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains aa1-504; ZF domain deleted (see note bel…

   Promoter

   pCAG

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pTAL_RFP

   Plasmid

   #43859

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330 NL-FKBP-Cas9

   Plasmid

   #162723

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   FKBP F36V
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTAL_GFP

   Plasmid

   #43858

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUDP012

   Plasmid

   #101167

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSOMA-T679A-NLS

   Plasmid

   #118938

   Purpose

   Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

   Depositor

   Patrick Krysan

   Article

   Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

   Insert

   SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-PHR-iSH-T2A-CIBNcaax

    Plasmid

    #173860

    Purpose

    Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

    Depositor

    Takao Nakata

    Article

    Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

    Insert

    mCherry-PHR-iSH-T2A-CIBNcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330 N-FKBP-Cas9

    Plasmid

    #162727

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. VIPR1

    Plasmid

    #51865

    Purpose

    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    VIPR1 (VIPR1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUG-T2A-Cas9

    Plasmid

    #75346

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid

    #140205

    Purpose

    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    mCherry
    sgRNA E1
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Tags

    Expression

    Mutation

    G174D

    Promoter

    PelcA and U3

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. L-CRISPR-CTN (MLL-ctrl)

    Plasmid

    #69215

    Purpose

    Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV promoter
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGMC00034 (aka pMC0235)

    Plasmid

    #210751

    Purpose

    Non-targeting guide RNA

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    Non-targeting guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SIN-PGK-Cas9-V5-WPRE

    Plasmid

    #87876

    Purpose

    To produce lentiviral vector for Cas9 editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Human codon-optimized

    Promoter

    mouse PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. myc-hRAD18 dSAP

    Plasmid

    #68830

    Purpose

    Expresses c-myc-tagged human RAD18 deleting SAP domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only