We narrowed to 20,958 results for: ACE

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NET33 pEGFP-N2 (597)

Plasmid

#61989

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET33 (SCARA5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7

Plasmid

#78176

Purpose

Expresses rsGreen0.7 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

Plasmid

#217725

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRE4

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-179

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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NET47 pEGFP-N2 (593)

Plasmid

#61986

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET47 (TM7SF2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.13/AUG-FFLuc-3XFLAG

Plasmid

#127333

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CV40

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(40nm)-Cys

Plasmid

#91571

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(40nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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AsdCas12a VPR

Plasmid

#188512

Purpose

Expresses FLAG tagged AsdCas12a VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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HsGRK6-mVenus

Plasmid

#137780

Purpose

Visualization of GRK6

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human GRK6 (GRK6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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phGR-SB

Plasmid

#133409

Purpose

overexpression of a human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Expression

Mammalian

Mutation

wt

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSCD-TVMVP

Plasmid

#226226

Purpose

Express TVMVP

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

TVMVP (TVMVgp1 Tobacco vein mottling virus)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPAkt

Plasmid

#184053

Purpose

Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-InPAkt

Tags

Lysosome-associated membrane protein 1 (LAMP1).

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PXN

Plasmid

#227315

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PXN (PXN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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L1_UBE2:mS-Lt-Ck3

Plasmid

#136131

Purpose

L1 in position 3, mark cell outline with mScarletI

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mScarletI-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Article

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Tags

yomRuby3

Expression

Yeast

Promoter

none

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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Affibody HER2:243.BirA*-pET301/NT-DEST

Plasmid

#174692

Purpose

Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

Depositor

Jean Gariepy

Article

Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

Insert

Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*

Tags

HIS tag

Expression

Bacterial

Mutation

R118G mutation in BirA

Promoter

T7 promoter

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTU422

Plasmid

#178982

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Inserts

gRAMP
CRISPR array

Tags

Strep-Tag II SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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L2383-FRB-GPA-NLuc

Plasmid

#222902

Purpose

Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTSAR3.4t

Plasmid

#210256

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

mCherry
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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L2_UBE2:eG-Lt-CsA

Plasmid

#136134

Purpose

Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpUBE2:eGFP-Lti6b

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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