We narrowed to 6,765 results for: sas

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PK2-Tango

Plasmid

#66479

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

PK2

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pGAD-hPLEKHM1

Plasmid

#64145

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

PLEKHM1 (PLEKHM1 Human)

Tags

GAL4 activation domain

Expression

Yeast

Promoter

ADH

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
Alpha 5 integrin-GFP

Plasmid

#15238

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

alpha 5 integrin (ITGA5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-CMV-bArrestin2-TEV

Plasmid

#107245

Purpose

Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ARRB2

Tags

TEV(NIA), tobacco etch virus NIA

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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SSTR5-Tango

Plasmid

#66506

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

SSTR5 (SSTR5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pHTNW

Plasmid

#136403

Purpose

Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pNLF1W

Plasmid

#136404

Purpose

Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MRGPRX2-Tango

Plasmid

#66440

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

MRGPRX2 (MRGPRX2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pAS_4xMx PylT_GFP 150TAG

Plasmid

#140016

Purpose

Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG, K46E (Please see depositor comments below)

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pFX-mito-at pHluorin

Plasmid

#174183

Purpose

Express EYFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

at pHluorin with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-Mma PylRS AF

Plasmid

#140023

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

Y271A, Y349F

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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TAAR2-Tango

Plasmid

#66509

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

TAAR2 (TAAR2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
TAAR5-Tango

Plasmid

#66510

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

TAAR5 (TAAR5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
TAAR6-Tango

Plasmid

#66511

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

TAAR6 (TAAR6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

Plasmid

#174890

Purpose

Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

U25C PylT

Promoter

EF1

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#215055

Purpose

Expresses EGFP in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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PLJR962

Plasmid

#115162

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-mCl3-iLID

Plasmid

#190165

Purpose

Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-mClover3-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ucp1-empty-miR122

Plasmid

#231695

Purpose

Empty control vector for pAAV-Ucp1-Cre-miR122

Depositor

Joerg Heeren

Article

Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

Type

Empty backbone

Use

AAV

Promoter

rat Ucp1 enhancer-promoter

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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EB3N-VVDfast-Halo-iLID

Plasmid

#190166

Purpose

Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

EB3N-VVDfast-Halo-iLID

Tags

-VVDfast-mClover3-iLID

Expression

Mammalian

Mutation

1-189 aa from EB3

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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