We narrowed to 22,833 results for: Sis

1. pLV-EF1a-GFP ArgSen

   Plasmid

   #184704

   Purpose

   Lentiviral transfer plasmid for expression of GFP ArgSen

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   GFP ArgSen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAd-Track Flag-SIRT1

   Plasmid

   #8438

   Depositor

   Pere Puigserver

   Article

   Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUPERIOR.puro-shp53

   Plasmid

   #38035

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

   Insert

   shp53 (TP53 Human)
   

   Use

   RNAi

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pET-Galectin-8 FL

   Plasmid

   #68805

   Purpose

   Express Galectin-8 FL protein in Bacterial cells

   Depositor

   Hyun Kyu Song

   Article

   Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

   Insert

   Galectin-8 (LGALS8 Human)
   

   Tags

   N-terminal 6-histidine-tag

   Expression

   Bacterial

   Mutation

   No mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pGK:HygroR-CMV:mKate2-B_UTR

   Plasmid

   #68480

   Purpose

   Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mKate2-B_UTR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "B" added to 3&#x…

   Promoter

   CMV and pGK

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. CFP-GAI(1-172)

   Plasmid

   #37309

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   contains aa 1-172

   Promoter

   CMV

   Available Since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

7. wt dynamin 1 pEGFP

   Plasmid

   #34680

   Depositor

   Sandra Schmid

   Article

   Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

   Insert

   dynamin 1ba (DNM1L Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pJMP1185

   Plasmid

   #119255

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 458 p3E-2A-EGFP-CAAXpA

   Plasmid

   #195968

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-EGFP-CAAX (membrane)
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJMP1358

    Plasmid

    #119277

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAL175

    Plasmid

    #22276

    Purpose

    Expresses YFP-PIF for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40

    Plasmid

    #179119

    Purpose

    Expresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Ezr sgRNA
    

    Use

    AAV

    Promoter

    U6

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS416Gal TDP43 WT YFP

    Plasmid

    #27447

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Yeast

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pMRX-IP/SECFP-hATG9A

    Plasmid

    #58995

    Purpose

    Expresses SECFP-hATG9A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 9A (PPARG Human)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUW-tetO-mCherry

    Plasmid

    #193060

    Purpose

    doxycycline-inducible expression of mCherry in mammalian cell

    Depositor

    Filipe Pereira

    Article

    Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

    Plasmid

    #52544

    Purpose

    Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. R26-H2B-EGFP HR donor vector

    Plasmid

    #137925

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC150-KEIMA-REEP5

    Plasmid

    #201928

    Purpose

    PiggyBac Vector expressing KEIMA-REEP5

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    REEP5 (REEP5 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP HDAC5 WT

    Plasmid

    #32211

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pYPQ166

    Plasmid

    #109328

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1-TK1

    Plasmid

    #100544

    Purpose

    mammalian expression of thymidine kinase 1

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

    Insert

    thymidine kinase 1 (TK1 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

    Plasmid

    #109320

    Purpose

    Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

    Depositor

    William Lagor

    Article

    Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT/Caggs-NRAS G12V/D38A

    Plasmid

    #221073

    Purpose

    Expresses NRAS G12V/D38A in mammalian cells

    Depositor

    Hidenori Ichijo

    Article

    Odawara et al Commun Biol. 2024 Jun 5;7(1):691. doi: 1

    Insert

    NRAS (NRAS Human)
    

    Expression

    Mammalian

    Mutation

    G12V/D38A

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJOG1368

    Plasmid

    #167301

    Purpose

    Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    FCY-UPP fusion gene
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCR3-FLAG-TRAF6-C70A

    Plasmid

    #43926

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    TNF Receptor-Associated Factor 6 (Traf6 Mouse)
    

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    C70A

    Available Since

    Jan. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYO1125

    Plasmid

    #235742

    Purpose

    Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    p40 PX domain (NCF4 Human)
    

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    aa 2-149 with Cys at 2

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCK082 desmaMCS:minprommCherry,exorh:mCerulean

    Plasmid

    #200015

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCherry, exorh:mCerulean

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NG-ABE8-dimer

    Plasmid

    #138497

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pICH47822_HA-Sr62TK

    Plasmid

    #233535

    Purpose

    Expresses HA-Sr62TK in planta

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62TK
    

    Tags

    HA tag

    Expression

    Plant

    Promoter

    35S

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pICH47822_Sr62NLRa

    Plasmid

    #233537

    Purpose

    Expresses Sr62NLRa in planta

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62NLRa
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-myc-hIFITM3-Y20A

    Plasmid

    #58462

    Purpose

    Expresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Tyrosine 20 to alanine

    Promoter

    CMV IE

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

33. Nanog-2A-GFP

    Plasmid

    #59994

    Purpose

    Inserts GFP immediately downstream of the Nanog coding region

    Depositor

    Rudolf Jaenisch

    Article

    Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2A-GFP

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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34. pKD184

    Plasmid

    #90953

    Purpose

    Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Expression

    Bacterial

    Promoter

    Bba_J23114, PltetO-1, and PphsA

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-delta90GFP

    Plasmid

    #26645

    Depositor

    Anjen Chenn

    Article

    Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

    Insert

    delta90 beta-catenin (Ctnnb1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deletion of first 90 aa of beta catenin

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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36. pKD4-Nter-APEX2

    Plasmid

    #112868

    Purpose

    Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

    Depositor

    Eric Cascales

    Article

    Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

    Insert

    APEX2
    

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJEC819

    Plasmid

    #190815

    Purpose

    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9-XTEN-rpoA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    XTEN (linker), followed by the N-terminal domain …

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMZ7

    Plasmid

    #223180

    Purpose

    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

    Depositor

    Paul Blainey

    Article

    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

    Type

    Empty backbone

    Use

    Chromosomal integration

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5/FRT/TO GFP HSPA1L

    Plasmid

    #19484

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA1L (HSPA1L Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge