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pMpGWB116

Plasmid

#68570

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pPPEM

Plasmid

#162467

Purpose

Destination Expression vector for monocot plant primer editing

Depositor

Adam Bogdanove

Article

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Insert

Cas9H840A

Tags

MLV reverse transcriptase

Expression

Plant

Mutation

Change histidine 840 to Alanine

Promoter

maize Ubi

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 CASTOR1-HA Cterm

Plasmid

#84487

Purpose

overexpression of CASTOR1 with HA tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pZS*11_4enh

Plasmid

#149423

Purpose

Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Inserts

rbcS-E9 enhancer
35S core enhancer
Cab-1 enhancer
AB80 enhancer

Tags

T120>A

Expression

Bacterial

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX303 GFP-PIK3C2B

Plasmid

#162001

Purpose

Lentiviral expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2B (PIK3C2B Human)

Use

Lentiviral

Tags

eGFP

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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Cathepsin S

Plasmid

#84694

Purpose

for expression of Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Promoter

unknown

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMBA334

Plasmid

#214746

Purpose

High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGWB114

Plasmid

#68568

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMP71-mGFP-LLO-mPMEL

Plasmid

#174608

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane GFP fused to LLO and mouse PMEL antigen

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB215

Plasmid

#68606

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged Citrine (Citri…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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Sf-U7snRNA-ESE-AS-NsiI

Plasmid

#190694

Purpose

Basic vector used for the expression of U7-based antisense oligonucleotides

Depositor

Pim Pijnappel

Article

van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

Insert

U7 snRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U7 promoter

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHRSin-IRES2-EmGFP_HPC-4 Ab light

Plasmid

#187359

Purpose

Lentiviral expression of HPC-4 antibody light chain

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

HPC-4 antibody light chain

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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p1553 CMV-EBNA1 (delta GGA)

Plasmid

#26648

Depositor

Bill Sugden

Article

Aiyar et al J Biol Chem. 1998 Dec 4. 273(49):33073-8

Insert

Epstein-Barr Nuclear Antigen 1 (EBNA-1 Epstein-Barr Virus)

Expression

Mammalian

Mutation

deletion of 224 amino acids of EBNA-1 (aa101-324 …

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pUb-tdTomato-LP-Neo-LP

Plasmid

#231509

Purpose

Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

tdTomato fluorescent protein

Use

Unspecified; Acanthamoeba castellanii neff strain

Promoter

Acanthamoeba polyubiquitin

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGAPDH-Cre-HYG

Plasmid

#231510

Purpose

Expresses Cre recombinase in Acanthamoeba

Depositor

Yeonchul Hong

Article

Aung et al Parasites Hosts Dis. 2025 Feb;63(1):25-3

Insert

Cre recombinase

Use

Unspecified; Acanthamoeba castellanii neff strain

Tags

EGFP and SV40 NLS

Promoter

Acanthamoeba GAPDH

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMBA333

Plasmid

#214745

Purpose

Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pZmUBQ:AVRa1-V1

Plasmid

#132357

Purpose

Expression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Article

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

AVRa1-V1 ∆SP with stop codon

Expression

Plant

Promoter

pZmUBQ

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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mVenus-PKCB1 K371R

Plasmid

#195230

Purpose

Retroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PKCB1 (PRKCB Human)

Use

Retroviral

Mutation

K371R

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pRW004-SM-destination-proUBQ10-Cas9

Plasmid

#104438

Purpose

Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

UBQ10

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB405

Plasmid

#68670

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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