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pET28B-PU.1-ETS-Wing

Plasmid

#124552

Purpose

Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

The 5 residues making up the "wing" in …

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET41a iGluSnFR E25R

Plasmid

#119831

Purpose

Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25R

Use

Tags

GST

Expression

Bacterial

Mutation

E25R

Promoter

T7

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pET-SOMA-T679A

Plasmid

#118936

Purpose

Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes7/Imdes7

Plasmid

#107115

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des7

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes7/Imdes7

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes8/Imdes8

Plasmid

#107116

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des8

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes8/Imdes8

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes9/Imdes9

Plasmid

#107117

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des9

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes9/Imdes9

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes10/Imdes10

Plasmid

#107118

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des10

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes10/Imdes10

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes11/Imdes11

Plasmid

#107119

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des11

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes11/Imdes11

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes12/Imdes12

Plasmid

#107120

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des12

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes12/Imdes12

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge