We narrowed to 11,680 results for: cel.2

1. Cdc2-DN-HA

   Plasmid

   #1889

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdc2 (CDK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D146N, dominant negative

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSBtet-2xMARS-nGFP-puro

   Plasmid

   #205239

   Purpose

   DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

   Depositor

   Jeremy Baskin

   Insert

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   

   Use

   Transposon

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   tight TRE promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Cdc2-DN

   Plasmid

   #1887

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdc2 (CDK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D146N, dominant negative

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCDH-2xMARS-nGFP-puro

   Plasmid

   #205238

   Purpose

   Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

   Depositor

   Jeremy Baskin

   Insert

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   

   Use

   Lentiviral

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFH2.19_mCherry-MS2x2

   Plasmid

   #205534

   Purpose

   Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-Rho*-BERKY3

   Plasmid

   #158220

   Purpose

   BRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-Gα13*-BERKY3

   Plasmid

   #158218

   Purpose

   BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PM PKA Reporter

   Plasmid

   #104551

   Purpose

   Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Lyn-mTurquoise2-VASP 148 - 164
   

   Use

   Tags

   mTurquoise 2, HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-IRES-Gβγ-BERKY3

   Plasmid

   #158426

   Purpose

   BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-IRES-Gαq*-BERKY3

    Plasmid

    #158425

    Purpose

    BRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-Hyg

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-Gαi*-BERKY3

    Plasmid

    #158198

    Purpose

    BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-Gαq*-BERKY3

    Plasmid

    #158217

    Purpose

    BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs-Brn2

    Plasmid

    #32925

    Purpose

    Retroviral expression of mouse Brn2

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Brn2 (Pou3f2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-Gαi*-BERKY1

    Plasmid

    #158090

    Purpose

    BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-KB1753-myc
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Gαi*-BERKY2

    Plasmid

    #158197

    Purpose

    BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-2xYFP-KB1753-myc
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Gα13(EE)-YFP

    Plasmid

    #171374

    Purpose

    Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Ga13(EE)-YFP
    

    Use

    Tags

    Glu-Glu

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-IRES-Gαi*-BERKY3

    Plasmid

    #158424

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+ Lifeact-mCherry

    Plasmid

    #185408

    Purpose

    Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    lifeact
    

    Use

    In vitro synthesis of mrna

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSG5-ERRbeta2

    Plasmid

    #52186

    Purpose

    Expresses human ERRbeta2 splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type sequence; codon optimized to exclude Ec…

    Promoter

    SV40

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225062

    Purpose

    Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225063

    Purpose

    Expression of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSG5-ERRbeta-delta10

    Plasmid

    #52187

    Purpose

    Expresses human ERRbeta-delta10 splice variant in mammalian cells

    Depositor

    Rebecca Riggins

    Article

    Heckler et al Cell Cycle. 2014 Dec 12:0.

    Insert

    Estrogen-related receptor beta (ESRRB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type sequence; codon optimized to exclude Ec…

    Promoter

    SV40

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

23. KRP85

    Plasmid

    #24448

    Depositor

    Ron Vale

    Article

    Pierce et al Biochemistry. 1999 Apr 27. 38(17):5412-2

    Insert

    Kinesin II (KRP85 Sea urchin)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

24. KRP95

    Plasmid

    #24447

    Depositor

    Ron Vale

    Article

    Pierce et al Biochemistry. 1999 Apr 27. 38(17):5412-2

    Insert

    Kinesin II (KRP95 Strongylocentrotus purpuratus)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pRB30

    Plasmid

    #165480

    Purpose

    pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-FLAG-10x His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRB33

    Plasmid

    #165482

    Purpose

    pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    10xHis-FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRB33-GFP

    Plasmid

    #165483

    Purpose

    pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal uv-GFP, Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    10xHis-FLAG-GFP-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FLT3-MSCV-IRES-GFP

    Plasmid

    #184778

    Purpose

    Expresses wildtype FLT3 in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EF1a_TBX5_P2A_Hygro_Barcode

    Plasmid

    #120486

    Purpose

    Barcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    TBX5 (TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EF1a_SIX2_P2A_Hygro_Barcode

    Plasmid

    #120477

    Purpose

    Barcoded lentiviral vector to express SIX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SIX2 (SIX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge