Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,565 results for: Tet-on

Clear keywords
Showing: 5241 - 5260 of 7565 results

  1. pUt-mNF-BACH1

    Plasmid
    #199219
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV D2ir promoter
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    199219-plasmid-map-sequence-id-396467

    Enlarge

  4. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199218-plasmid-map-sequence-id-396470

    Enlarge

  5. pIND21-puro_SOX10-OLIG2-NKX6.2-3xFLAG

    Plasmid
    #250320
    Purpose
    Tetracycline-inducible expression of SOX10-OLIG2-NKX6.2 as polyprotein linked by self cleavage sites P2A and T2A. rtTA (Tet-ON) is driven the EF1alpha promoter.
    Depositor
    Michael Wehr
    Article
    Raabe et al Cells. 2022 Jan 11;11(2):241. doi: 10.33
    Insert
    SOX10-P2A-OLIG2-T2A-NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    TRE2
    Available Since
    Feb. 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    250320-plasmid-map-sequence-id-501231

    Enlarge

  6. pHis-TEV-Nsp15

    Plasmid
    #187656
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004
    Insert
    Nsp15 (N Severe acute respiratory syndrome coronavirus 2)
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7lac
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    187656-plasmid-map-sequence-id-388768

    Enlarge

  7. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    van Voorst et al bioRxiv [Preprint]. 2025 Jul 7:2025.06.3
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and pTRE3G-bidirectional
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

    Enlarge

  16. pTRIPZ-FFSS-Cyclin D1 (HP)

    Plasmid
    #215150
    Purpose
    Expresses FFSS-Cyclin D1 (HP) in mammalian cells from an inducible lentiviral vector.
    Depositor
    Michele Pagano
    Article
    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012
    Insert
    CCND1 (CCND1 Human)
    Use
    Lentiviral
    Tags
    FFSS (FLAG-FLAG-STREP-STREP)
    Expression
    Mammalian
    Mutation
    Cyclin D1(HP) denotes mutations M56A, V60A, and W…
    Promoter
    CMV (Tet inducible)
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215150-plasmid-map-sequence-id-428730

    Enlarge

  17. dRED

    Plasmid
    #139836
    Purpose
    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    RNase H1 protein contains D210N mutation. dCas9 …
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139836-plasmid-map-sequence-id-308228

    Enlarge

  18. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Article
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139835-plasmid-map-sequence-id-310615

    Enlarge

  20. pCW-eGFP-FHA

    Plasmid
    #114129
    Purpose
    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    aa 2-160
    Promoter
    TRE promoter, Tet ON
    Available Since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114129-plasmid-map-sequence-id-220207

    Enlarge

Showing: 5241 - 5260 of 7565 results