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Plasmid#206957PurposeFluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2DepositorUseTagscSURF, mCherry, and nSURFExpressionMammalianMutation17-615 and RBD (319-541)PromoterCMVAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
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RED
Plasmid#139835PurposeExpresses RNase H1-EGFP- dCas9 fusion protein in human cellsDepositorInsertRNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
UseTagsExpressionMammalianMutationdCas9 protein contains two mutations: D10A and H8…Promotertet-inducible CMVAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
dRED
Plasmid#139836PurposeExpresses dRNase H1-EGFP-dCas9 fusion protein in human cellsDepositorInsertdRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
UseTagsExpressionMammalianMutationRNase H1 protein contains D210N mutation. dCas9 …Promotertet-inducible CMVAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK2-R67S/S153D
Plasmid#213597PurposeDoxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cellsDepositorInsertMAPK1 (MAPK1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR67S, S153DPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12D
Plasmid#81651PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12DPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_WT
Plasmid#81923PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_IDH1_p.R132C
Plasmid#81726PurposeGateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.DepositorInsertIDH1 (IDH1 Human)
UseGateway entry vectorTagsExpressionMutationR132CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GAPDH
Plasmid#83910Purposestable overexpressionDepositorInsertglyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_IDH2_p.R172K
Plasmid#81432PurposeGateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.DepositorInsertIDH2 (IDH2 Human)
UseGateway entry vectorTagsExpressionMutationR172KPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAPK1_WT
Plasmid#82145PurposeGateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.DepositorInsertMAPK1 (MAPK1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FBXW7_WT
Plasmid#81795PurposeGateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.DepositorInsertFBXW7 (FBXW7 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12C
Plasmid#81667PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-TAF15 Stop
Plasmid#84895PurposeDONOR vector for Gateway cloning of TAF15DepositorInsertTAF15 (TAF15 Human)
UseGateway donorTagsExpressionMutationPromoternoneAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTripZ RB1 WT-3XFLAG
Plasmid#209070PurposeDOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tagDepositorInserthuman RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
UseLentiviralTags3XFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTNNB1_p.S33A_S37A_T41A_T45A
Plasmid#82176PurposeGateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection. (Please note this plasmid contains S45A mutation, not T45A)DepositorInsertCTNNB1 (CTNNB1 Human)
UseGateway entry vectorTagsExpressionMutationS33A, S37A, T41A, S45APromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_AKT1_p.E17K
Plasmid#82089PurposeGateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.DepositorInsertAKT1 (AKT1 Human)
UseGateway entry vectorTagsExpressionMutationE17KPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HIF1A_WT
Plasmid#82129PurposeGateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.DepositorInsertHIF1A (HIF1A Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_TCF4_WT
Plasmid#82166PurposeGateway Donor vector containing TCF4 , part of the Target Accelerator Plasmid Collection.DepositorInsertTCF4 (TCF4 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12S
Plasmid#81654PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_WBSCR17_WT
Plasmid#81837PurposeGateway Donor vector containing WBSCR17 , part of the Target Accelerator Plasmid Collection.DepositorInsertWBSCR17 (GALNT17 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only