We narrowed to 9,108 results for: control

1. mScarlet-I-T2A-LSS-SGFP2

   Plasmid

   #112946

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Insert

   mScarlet-I and LSS-SGFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-GFP-miRE_shRen-Blast

   Plasmid

   #135684

   Purpose

   Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-tdTomato-miRE_shRen-Blast

   Plasmid

   #135686

   Purpose

   Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6:3-tevopreq1

   Plasmid

   #190909

   Purpose

   Expression of single epegRNA under control of Drosophila U6:3 promoter. Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Insert

   Empty Backbone
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTcTurboID-GW_GFP

   Plasmid

   #234715

   Purpose

   Used to express GFP fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling cytoplasmatic spatial control. CDS can be cloned in frame to GFP using EcoRV.

   Depositor

   Esteban Serra

   Insert

   GFP
   

   Use

   Trypanosomatid expression

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET41-FeoB-D123N

   Plasmid

   #216754

   Purpose

   Production of a FeoB that is unable to bind GTP for a negative control in GTPases assays

   Depositor

   Rietie Venter

   Insert

   feoB (PA4358 P. aeruginosa (Bacteria))
   

   Use

   Tags

   8x His

   Expression

   Bacterial

   Mutation

   Amino acid D123 is substituted with N.

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-GFP-miRE_shTHUMPD3-Blast

   Plasmid

   #135690

   Purpose

   Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   shRosa26
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-GFP-miRE_shTHUMPD3-Puro

   Plasmid

   #135691

   Purpose

   Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Puromycin selection

   Depositor

   Florian Karreth

   Insert

   shRosa26
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-GFP-miRE_shSCR-Blast

   Plasmid

   #135688

   Purpose

   Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   shScrambled
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-CMV-GFP-miRE_shRen-Puro

    Plasmid

    #135685

    Purpose

    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection

    Depositor

    Florian Karreth

    Insert

    shRenilla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.23-5xE-box

    Plasmid

    #206841

    Purpose

    The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

    Depositor

    Tassula Proikas-Cezanne

    Article

    Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

    Insert

    5x E-box ((GCCACGTGCA)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-OptoFGFR1

    Plasmid

    #59776

    Purpose

    Expresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused protein

    Depositor

    Won Do Heo

    Article

    Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

    Insert

    OptoFGFR1 (FGFR1 Human, Mustard Weed)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IP-GFP-LC3-RFP

    Plasmid

    #84573

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-IP-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #84572

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

    Plasmid

    #218857

    Purpose

    Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

    Depositor

    Marnie Halpern

    Article

    Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

    Insert

    hGCGR (GCGR Human)
    

    Use

    Tags

    QF fusion protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-TK-5UTR-BsmBI-Luciferase

    Plasmid

    #114670

    Purpose

    For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

    Depositor

    Lynne Postovit

    Article

    Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

    Insert

    LacZ
    

    Use

    Luciferase

    Tags

    for cloning 5' UTR-Luciferase fusions

    Expression

    Mammalian

    Mutation

    Promoter

    lac promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ARHGEF11(DHPH)-CRY2-mCherry

    Plasmid

    #89481

    Purpose

    Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11

    Depositor

    Xavier Trepat Guixer

    Article

    Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

    Insert

    ARHGEF11(DHPH) - CRY2(1-498) - mCherry (CRY2, ARHGEF11 Human, Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-flag S127A YAP

    Plasmid

    #27370

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    S127A ( major phosphorylation site in YAP2)

    Promoter

    Available since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV 3xFLAG-UBR5

    Plasmid

    #231238

    Purpose

    Flag-tagged wild-type UBR5 over-expression construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

    Insert

    UBR5 (UBR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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