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pNLF1-N_CORO1A:1-461

Plasmid

#210964

Purpose

Mammalian expression of full-length human CORO1A. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1A:M1-K461 (CORO1A Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CORO1A:1-461

Plasmid

#210965

Purpose

Mammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1A:M1-K461 (CORO1A Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DCAF1:1038-1400

Plasmid

#210962

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_FBXW11:1-542

Plasmid

#210952

Purpose

Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW11:M1-R542 (FBXW11 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_SKIC8:1-305

Plasmid

#211038

Purpose

Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SKIC8:M1-I305 (SKIC8 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_COPB2:1-597

Plasmid

#211055

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-A597 (COPB2 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTS26_QUAS_CsChrimson

Plasmid

#175548

Purpose

Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

Depositor

Leslie Vosshall

Article

Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

Insert

CsChrimson

Tags

tdTomato

Expression

Insect

Promoter

QUAS

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-C1

Plasmid

#60560

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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CRY2olig-mCherry

Plasmid

#60032

Purpose

Expresses a fusion of CRY2PHR E490G with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2PHR E490G (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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SpyCas9-T1048-cpGR2(#4670)

Plasmid

#250780

Purpose

Potent chemically-activated and temperature-deactivated SpyCas9

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

SpyCas9 with a cpGR2 insertion behind T1048

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pBABE puro human IRS-1

Plasmid

#11025

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

Insert

IRS-1 (IRS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT1

Plasmid

#53554

Purpose

mammalian expression of IFIT1

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-mC-S

Plasmid

#172427

Purpose

Expression of full-length, human GIRK4 with C-terminal mCherry StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

mCherry Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-S

Plasmid

#172425

Purpose

Expression of full-length, human GIRK4 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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CMV500 A-FOS

Plasmid

#33353

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

FOS (Fos Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative of c-Jun; heterodimerizes and i…

Promoter

CMV

Available Since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

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CRY2olig(FL)-mCherry

Plasmid

#60033

Purpose

Expresses a fusion of full length CRY2 (E490G) with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2 E490G (deltaNLS) (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G, NLS mutated

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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mRFP-Lact-C2

Plasmid

#74061

Purpose

Fluorescent biosensor for phosphatidylserine.

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 (MFGE8 Bovine)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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