We narrowed to 143,240 results for: ins

1. pET28a Gx-NL2

   Plasmid

   #169107

   Purpose

   Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - NanoLuc - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Vh-HA-2xFKBP

   Plasmid

   #20221

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   2xFKBP (FKBP1A Human)
   

   Use

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

3. PKG I beta CAT

   Plasmid

   #16396

   Depositor

   Bernard Weinstein

   Article

   Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

   Insert

   PKG1 beta (PRKG1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   CAT: catalytic domain

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

4. Hy_pMT Laccase2 MCS-WT Split GFP

   Plasmid

   #69891

   Purpose

   Expresses a GFP circular RNA in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   GFP, laccase2 (stw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIC389

   Plasmid

   #37387

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   LGN (GPSM2 Human)
   

   Use

   Retroviral

   Tags

   GFP, S-tag, and TEV

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-364 and 524

   Promoter

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 3xFLAG TPP1 88-249

   Plasmid

   #53549

   Purpose

   mammalian expression of TPP1 OB-fold domain

   Depositor

   Kathleen Collins

   Article

   Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   aa 88-249

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CaMKIIa-ChRger3-TS-EYFP

   Plasmid

   #127241

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger3-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIa

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMTH PKC beta II

   Plasmid

   #8410

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Rat Protein Kinase C beta II (Prkcb Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

   Plasmid

   #91873

   Purpose

   Photo-activatable/light sensitive Vav2

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   VAV2 DH/PH/ZF (Vav2 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX Stat4-SH2

    Plasmid

    #46514

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat4 (STAT4 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 133-678

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX Slap-SH2

    Plasmid

    #46500

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Slap (SLA Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 61-221

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Syk(C)-SH2

    Plasmid

    #46519

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Syk(C) (SYK Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 149-263

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac1-OSF-TRIM5alpha(rhesus)

    Plasmid

    #79033

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(rhesus)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

    Plasmid

    #185711

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed I770 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX Vav1-SH2

    Plasmid

    #46528

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Vav1 (VAV1 Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 661-777

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C. difficile toxin B mRNA toehold switch sensor

    Plasmid

    #110715

    Purpose

    Toehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    C. difficile toxin B toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B. fragilis mRNA toehold switch sensor

    Plasmid

    #110706

    Purpose

    Toehold switch sensor to detect a species specific mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis species specific toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABE bleo mouse IRS-2

    Plasmid

    #11447

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pCarCBA

    Plasmid

    #117754

    Purpose

    Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

    Depositor

    Greg Bokinsky

    Article

    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

    Insert

    Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac(UV5)

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-CmTMC1truncated

    Plasmid

    #154908

    Purpose

    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

    Depositor

    Motoyuki Hattori

    Article

    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

    Insert

    GFP-CmTMC1truncated
    

    Use

    Tags

    8histidine-EGFP

    Expression

    Insect

    Mutation

    Truncations of the residues 1-243 and 843-969

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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