20,053 results

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pCMV 3xFLAG-LC3C WT

Plasmid

#123095

Purpose

Expresses 3xFLAG-LC3C wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hM4D(Gi)-mCherry

Plasmid

#50477

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has Service

AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

hM4D(Gi)-mCherry (CHRM4 Human)

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

CaMKIIa

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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p16L1L2

Plasmid

#45291

Depositor

John Schiller

Article

Buck et al Curr Protoc Cell Biol. 2007 Dec;Chapter

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

EM7

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pNAHA

Plasmid

#44169

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

hemagglutinin gene
neuraminidase gene

Expression

Mammalian

Mutation

T55A

Promoter

CMV enhancer/human Ferritin light chain promoter …

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAP

Plasmid

#166457

Purpose

Expresses fusion of mEGFP and YAP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAP (YAP1 Human)

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpe

Plasmid

#13787

Purpose

Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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FUW-ubiquitin-SV40-GFP

Plasmid

#65445

Purpose

Expression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectors

Depositor

Eduard Batlle

Article

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

SV40-EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

UBQ and SV40

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
JD553

Plasmid

#160392

Purpose

Wheat GRF4-GIF1 chimera in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

wheat GRF4-GIF1 chimera

Use

Entry clon

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

Plasmid

#101852

Purpose

Direct reprogramming of adult fibroblasts to neurons

Depositor

Malin Parmar

Article

Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

Insert

Ascl1, Brn2

Use

Lentiviral

Promoter

U6, PGK

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-Rab5

Plasmid

#49201

Purpose

expression of Rab5 fused to mCherry

Depositor

Gia Voeltz

Article

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

Rab5 (RAB5B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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LRRK2-WT

Plasmid

#17609

Depositor

Ted Dawson

Article

West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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PEmax-mRNA

Plasmid

#204472

Purpose

plasmid for PEmax mRNA IVT

Depositor

Scot Wolfe

Article

Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

Insert

Prime editor

Use

CRISPR

Tags

no

Expression

Mammalian

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-O2S

Plasmid

#21162

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4, Sox2 (SOX2, POU5F1 Human)

Use

Lentiviral; Reprogramming

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-VGAT-WPRE-pA

Plasmid

#39320

Depositor

Bernardo Sabatini

Article

Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

Insert

Slc32a1 (Slc32a1 Mouse)

Use

AAV; R

Available Since

Oct. 9, 2012

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT

Plasmid

#1787

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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GFP-BAZ2A

Plasmid

#65373

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BAZ2A (BAZ2A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-TREX1(D18N)

Plasmid

#27220

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

D18N sequence 52-GAC changed to AAC, resulting i…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pCIRCNpu

Plasmid

#74226

Purpose

Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

Npu DnaE C-intein and Npu DnaE N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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